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ABS1631 Anti-phospho-eNOS (Ser602)

Detect endothelial Nitric oxide synthase using this rabbit polyclonal Anti-phospho-eNOS (Ser602), Cat. No. ABS1631. It has been tested for use in ELISA and Western Blotting.

Anti-phospho-eNOS (Ser602) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: NOS3 UniProt Number: P29473
ABS1631
100 µL  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Species ReactivityKey ApplicationsHostFormatAntibody Type
BWB, ELISARbPurifiedPolyclonal Antibody
Description
Catalogue NumberABS1631
DescriptionAnti-phospho-eNOS (Ser602)
Alternate Names
  • Nitric oxide synthase endothelial
  • Constitutive NOS
  • EC-NOS
  • eNOS
  • NOS III
  • NOS type III
Background InformationNitric oxide synthase, endothelial (UniProt: P29473; also known as EC:1.14.13.39; constitutive NOS, EC-NOS, eNOS, NOS III, NOS type III) is encoded by the NOS3 gene (Gene ID: 287024) in bovine. The eNOS is mainly expressed in vascular endothelial cells and cardiomyocytes. It produces nitric oxide (NO) that is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. In endothelial cells, eNOS is localized in specialized plasmalemmal pockets known as caveolae, which contain a scaffolding protein termed caveolin. Acylation of eNOS by either myristate or palmitate targets it to caveolae, which facilitates enzyme activation following receptor stimulation. In a resting cell, eNOS is inhibited by its interaction with caveolin. Following agonist activation, eNOS dissociates from caveolin and translocates to the cytosol. The eNOS is generally activated in response to an increase in intracellular Ca2+ following stimulation with receptor-dependent agonists such as acetylcholine. However, shear stress can also activate this enzyme independently of Ca2+ level changes, via a tyrosine kinase-dependent mechanism involving its phosphorylation and redistribution. Perturbations of eNOS phosphorylation have been reported in a number of diseases. The primary sites where eNOS gets phosphorylated are multiple serine residues and, to a lesser extent, on tyrosine and threonine residues. Ser 602 is reported to lie at the N-terminal edge of the 42-residue eNOS autoinhibitory element and is phosphorylated by ERK. Low concentrations of nitric oxide in the endothelial cells prevent their apoptotic death and maintain their integrity during inflammation and atherosclerosis. Distorted production of nitric oxide in endothelial cells has been linked to hypertension, hypercholesterolemia, diabetes, and even cardiac failure. (Ref.: Rafikov, R., et al. (2011). J. Endocrinol. 210(3): 271-284).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in PBS with 0.02% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect endothelial Nitric oxide synthase using this rabbit polyclonal Anti-phospho-eNOS (Ser602), Cat. No. ABS1631. It has been tested for use in ELISA and Western Blotting.
Key Applications
  • Western Blotting
  • ELISA
Application NotesWestern Blotting Analysis: A representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysate (BAEC) (Salerno, J.C. et. al. (2014). Biosci Rep. 34(5). pii: e00137).

Western Blotting Analysis: A 1:250-1,000 dilution from a representative lot detected phospho-eNOS (Ser602) in bovine aortic endothelial cell lysates (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).

ELISA Analysis: A 1:64,000 dilution from a representative lot detected phospho-eNOS (Ser602) in Phospho-602 peptide/Non-Phospho-602 peptide (Courtesy of Dr. Carol Chrestensen at Kennesaw State University, Kennesaw, GA).
Biological Information
ImmunogenA linear peptide of 11 amino acids from the N-terminal edge of the 42-residue bovine eNOS autoinhibitory element.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis polyclonal antibody specifically detects the phospho-Ser602 in eNOS. It targets a sequence of 11 amino acids surrounding Ser602.
Species Reactivity
  • Bovine
Species Reactivity NoteBovine. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NOS3
Modifications
  • Phosphorylation
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~150 kDa observed; 133.29 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in bovine aortic endothelial cell lysates.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-eNOS (Ser602) in 10 µg of bovine aortic endothelial cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
ABS1631 04054839174452