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SCT025 BioTracker 519 Green β-Gal Dye

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

BioTracker 519 Green β-Gal Dye MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Cell Based Assays
SCT025
5x30nmol  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

Key Applications
Cell Based Assays
Description
Catalogue NumberSCT025
DescriptionBioTracker 519 Green β-Gal Dye
Overviewβ-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).

The BioTracker 519 Green β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. The dye can stain LacZ expressing HEK293 cells both before and after 3% paraformaldehyde fixation.

Spectral Properties
Absorbance: 497nm
Emission: 519nm
Alternate Names
  • GlycoGREEN™–βGal
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationLive cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker 519 Green β-Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening
Packaging Information
Material Size5x30nmol
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SCT025 04054839545580