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SCT251 BioTracker BSH Activity Bioluminescent Probe


This BSH-activatable luciferin (BAL) probe provides a noninvasive optical readout-based method for quantifying BSH activity. Biological applications include measurement of BSH activity of pure enzyme, or diverse samples such as live bacteria, fecal samples, or through the use of in vivo noninvasive imaging.

SCT251
1 mg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberSCT251
DescriptionBioTracker BSH Activity Bioluminescent Probe
OverviewReference
Sci Adv. 2021, 7(6): eaaz9857.
Alternate Names
  • Bile Salt Hydrolase probe
  • BSH probe
  • BSH bioluminescent probe
  • BSH cell and tissue probe
Background InformationThe human gut microbiome and its role in both health and disease has been studied extensively, laying the foundation for its involvement in metabolism, nutrition, physiology, and immune function. Bile salt hydrolase (BSH), an enzyme produced by gut bacteria, cleaves an amide bond in conjugated bile acids that results in formation of glycine and taurine molecules as well as secondary bile acids. These are important in host lipid and glucose metabolism as well as gut microbe composition, providing necessary components for gastrointestinal microbe survival. Secondary bile acids also play a role in a variety of health conditions such as colon cancer and age-related diseases relevant to life-span. Increased BSH activity has positive benefits in a variety of areas such as reduced inflammation, reduced cholesterol levels, improved protection against colon cancer, reduction of urinary tract infections, and even improved symptoms for patients with Crohn’s disease. However, the mechanisms by which BSH function is regulated are poorly understood, and knowledge is limited by lack of noninvasive detection methods amenable to use within an intact gut microbiome environment.
References
Product Information
Detection methodBioluminescence Luminometry
FormatLyophilized solid
PresentationLyophilized. Yellow solid.
Quality LevelMQ100
Applications
Application
This BSH-activatable luciferin (BAL) probe provides a noninvasive optical readout-based method for quantifying BSH activity. Biological applications include measurement of BSH activity of pure enzyme, or diverse samples such as live bacteria, fecal samples, or through the use of in vivo noninvasive imaging.
Key Applications
  • Activity Assay
  • Cell Based Assays
  • Microbial monitoring
  • Enzyme Assays
Application NotesBioluminescent enzyme activity measurement in cells and tissues
Biological Information
Molecular Weight653.89
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Purity: ≥ 97% confirmed by HPLC, HNMR, LC-MS and elemental analysis. Molar Mass: 653.89 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store BioTracker™ Bile Salt Hydrolase Bioluminescent Probe at -20°C, desiccated and protected from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SCT251 04065268463642