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14-448 Cdk2/Cyclin A Protein, active, 10 µg

Active, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.

Cdk2/Cyclin A Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-448
10 µg  Also available in 250μg size, please inquire for pricing and availability.
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-448
Replaces14-407
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk2/Cyclin A Protein, active, 10 µg
OverviewC-terminal His6-tagged human full length cdk2, and N-terminal GST-tagged human full length cyclin A
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, C-terminal His6-tagged human full length Cdk2 & N-terminal GST-tagged human full length Cyclin A, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed individually by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Gene Symbol
  • CDK2
  • p33(CDK2)
Protein TargetCDK2/cyclinA
Purification MethodThe cdk2 is purified by Ni2+/NTA agarose and then activated using CAK and repurified by Q Sepharose™ and Ni2+/NTA agarose. The cyclin A is purified using glutathioneagarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P24941 # Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2.
SIZE: 298 amino acids; 33930 Da
SUBUNIT: Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1 (By similarity). Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1.
SIMILARITY: SwissProt: P24941 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight35kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size, please inquire for pricing and availability.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-448 04053252278143

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产品目录编号 描述
14-448M Cdk2/Cyclin A Protein, active, 250 µg

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases