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14-477M Cdk5/p35 Protein, active, 250 µg

Active complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.

Cdk5/p35 Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-477M
250 µg  Also available in 10 μg size (cat#14-477) and in bulk (cat# 14-477-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-477M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionCdk5/p35 Protein, active, 250 µg
OverviewComplex of recombinant fulllength human cdk5 containing and N-terminal His6-tag and recombinant full-length human p35 containing an N-terminal GST-tag
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive complex of recombinant full length human Cdk5 containing & N-terminal His6-tag & recombinant full-length human p35 containing an N-terminal GST-tag, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceBoth are expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • CDK5
  • PSSALRE
Protein TargetCDK5/p35
Purification MethodNi-NTA agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q00535 # Probably involved in the control of the cell cycle. Interacts with D1 and D3-type G1 cyclins. Can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. Also interacts with p35 which activates the kinase.
SIZE: 292 amino acids; 33304 Da
SUBUNIT: Heterodimer of a catalytic subunit and a regulatory subunit (p35). Found in a trimolecular complex with CABLES1 and ABL1. Interacts with CABLES1 (By similarity). Interacts with AATK.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, growth cone (By similarity). Note=In axonal growth cone with extension to the peripheral lamellipodia (By similarity).
SIMILARITY: SwissProt: Q00535 ## Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. & Contains 1 protein kinase domain.
Molecular Weight34kDa (cdk5), 61kDa (p35 with GST-tag)
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Histone H1
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-477) and in bulk (cat# 14-477-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-477M 04053252284298

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-477 Cdk5/p35 Protein, active, 10 µg

产品族

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases