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344146 FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli

FPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

同义词: FTase, Farnesyl Proteintransferase

344146
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概述

Replacement Information

Products

产品目录编号包装 数量 / 包装
344146-100UGCN 塑胶安瓿;塑胶针药瓶 100 μg
Description
OverviewRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
Catalogue Number344146
Brand Family Calbiochem®
SynonymsFTase, Farnesyl Proteintransferase
References
ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
Product Information
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Quality LevelMQ100
Applications
ApplicationFPTase, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, is composed of an74 kDa α- and 47 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence.
Biological Information
Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Purity≥90% by SDS-PAGE
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
344146-100UGCN 04055977215236

Documentation

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli MSDS

职位

物料安全数据表 (MSDS) 

FPTase, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli 分析证书

标题批号
344146

参考

参考信息概述
Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.
数据表

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-June-2008 RFH
SynonymsFTase, Farnesyl Proteintransferase
DescriptionRecombinant, rat FPTase composed of an α- and a β-subunit (74 and 46 kDa, respectively). The α-subunit is expressed as a GST-fusion protein with an N-terminal His•Tag® sequence. Both the GST moiety and the His•Tag® sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ and Mg2+ for optimal activity.
FormLiquid
FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Purity≥90% by SDS-PAGE
Biological activity1 pmol FTase will transfer ≥1 pmol farnesyl to H-Ras in 10 min at 37°C, pH 7.2.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesZimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
Reiss, Y., et al. 1990. Cell 62, 81.