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14-610M Flt-3 (D835Y) Protein, active, 250 µg

Active, N-terminal GST tagged, recombinant human Flt3 amino acids 564-end, containing the mutation D835Y, for use in Kinase Assays.

Flt-3 (D835Y) Protein, active, 250 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-610M
250 µg  Also available in 10 μg size (cat#14-610) and in bulk (cat# 14-610-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-610M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionFlt-3 (D835Y) Protein, active, 250 µg
OverviewN-terminal GST tagged, recombinant human Flt3 amino acids 564-end, containing the mutation D835Y
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, N-terminal GST tagged, recombinant human Flt3 amino acids 564-end, containing the mutation D835Y, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. The receptor consists of an extracellular domain composed of five immunoglobulin-like domains, one transmembrane region, and a cytoplasmic kinase domain split into two parts by a kinase-insert domain. The receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia.
Gene Symbol
  • FLT3
  • STK-1
  • FLK2
  • CD135
  • OTTHUMP00000042340
  • STK1
Protein TargetFlt3
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P36888 # Receptor for the FL cytokine. Has a tyrosine-protein kinase activity.
SIZE: 993 amino acids; 112903 Da
SUBUNIT: Interacts with FIZ1 following ligand activation (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Bone marrow cells.
SIMILARITY: SwissProt: P36888 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 1 Ig-like C2-type (immunoglobulin-like) domain. & Contains 1 protein kinase domain.
Molecular Weight77.4kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of 50μM Abltide (Catalog # 12-493)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-610) and in bulk (cat# 14-610-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-610M 04053252508783