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IPVH304F0 Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm

Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析

Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
主要应用: 西方蛋白印迹
IPVH304F0
10  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

主要应用
西方蛋白印迹
描述
产品目录编号IPVH304F0
商名
  • Immobilon
描述Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm
背景信息Overview
- Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

Immobilon-P Transfer Membrane:
- 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
- Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

Immobilon-E Membrane:
- the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
- 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

Blotting Sandwiches:
- When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

Blotting Filter Paper:
- Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

Immobilon-PSQ Membrane:
- 0.2 µm pore size and a large internal structure
- Higher protein adsorption and sequencing yields than other membranes
- Recommended for blotting proteins less than 20 kDa
- Prevents blow-through of low molecular weight proteins

Immobilon-FL Membrane:
- The first transfer membrane optimized for fluorescence applications
- Extremely low background improves sensitivity of all fluorescence detection protocols
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
- Ideal for multiplexing and chemifluorescence applications

Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

Features & Benefits:
- Won’t crack, curl or fracture when cut
- Low background
- Superior staining capabilities
- Can be reprobed multiple times

Applications:
- Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
- Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
- Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
参考
产品信息
检测方法呈色 放射
相容的染色剂
  • 考马斯亮蓝
  • 酰胺黑
  • 印度墨水
  • 丽春红
  • 胶体金
  • CPTS
  • 甲苯胺蓝
  • 透射显影
  • Sypro® 宝石红
过滤器代码IPVH
过滤器颜色白色
Quality LevelMQ400
应用
应用Western blotting、吸附分析、氨基酸分析、N 端蛋白质测序、Dot/Slot blotting、糖蛋白显色和脂多糖分析
主要应用
  • 西方蛋白印迹
生物信息
主结合机制静电，疏水
BSA吸收性215 µg/cm²
山羊IgG吸收性294 µg/cm²
胰岛素吸收性160 µg/cm²
可湿润性疏水
理化信息
孔径0.45 µm
尺寸
过滤器表面平纹
过滤器尺寸26 cm x 26 cm
材料信息
化学
  • 疏水性聚偏氟乙烯（PVDF）
毒性学信息
GHS安全信息
安全信息
产品使用声明
储存和货运信息
包装信息
数量10
运输信息
补充信息
规格
Global Trade Item Number
产品目录编号 GTIN
IPVH304F0 04053252471339

Documentation

Immobilon-P 方片膜，PVDF，0.45 µm，26 x 26 cm MSDS

职位

物料安全数据表 (MSDS) 

参考

参考概述应用
Quantitation of Protein on gels and blots by infrared fluorescence of Coomassie blue and fast green
Luo S., Wehr N.B., Levine R.L.
Analytical Biochemistry:350 (2006):233-238  2006

Immunoblotting (Western)

小册子

标题
Western Blotting Tools

技术信息

标题
Protein Blotting Handbook: 6th Edition

数据表

标题
Immobilon Transfer Membranes: For superior protein and nucleic acid blots

用户指南

标题
Immobilon -P Blotting Sandwiches
Immobilon®-P Transfer Membrane User Guide