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14-596M LKB1/STRADα/MO25α Protein, active, 125 µg

Active, recombinant full-length human LKB1 with an N-terminal 6His tag. Provided as a complex with GST-STRADα & GST-MO25α. For use in Kinase Assays.

14-596M
125 µg  Also available in 10 μg size (cat#14-596) and in bulk (cat# 14-596-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-596M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionLKB1/STRADα/MO25α Protein, active, 125 µg
OverviewRecombinant full-length human LKB1 with an N-terminal 6His tag. Provided as a complex with GST-STRADα and GST-MO25α.
References
Product Information
Quality LevelMQ100
Applications
ApplicationActive, recombinant full-length human LKB1 with an N-terminal 6His tag. Provided as a complex with GST-STRADα & GST-MO25α. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in Sf21
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.
Gene Symbol
  • STK11
  • PJS
  • LKB1
Protein TargetLKB1
Purification MethodGSH agarose followed by Ni2+/NTA agarose
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q15831 # Essential role in G1 cell cycle arrest. Phosphorylates and activates members of the AMPK-related subfamily of protein kinases. Tumor suppressor.
COFACTOR: Magnesium or Manganese.
SIZE: 433 amino acids; 48636 Da
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Relocates to the cytoplasm when bound to CAB39 and STRAD or CAB39 and ALS2CR2.
TISSUE SPECIFICITY: Ubiquitously expressed. Strongest expression in testis and fetal liver.
PTM: Phosphorylated by a cAMP-dependent protein kinase (By similarity).
DISEASE: SwissProt: Q15831 # Defects in STK11 are a cause of Peutz-Jeghers syndrome (PJS) [MIM:175200]. PJS is a rare hereditary disease in which there is predisposition to benign and malignant tumors of many organ systems. PJS is an autosomal dominant disorder characterized by melanocytic macules of the lips, multiple gastrointestinal hamartomatous polyps and an increased risk for various neoplasms, including gastrointestinal cancer. & Defects in STK11 have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: Q15831 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight51/76/66kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of LKBtide.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size125 µg
Material PackageAlso available in 10 μg size (cat#14-596) and in bulk (cat# 14-596-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-596M 04053252277306

Documentation

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产品目录编号 描述
14-596 LKB1/STRADα/MO25α Protein, active, 10 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases