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14-586 MAPKAP Kinase 3 Protein, unactive, 50 µg

Unactive, N-terminal, GST-tagged, recombinant, human MAPKAP Kinase 3, amino acids 2-end, for use in Kinase Assays.

MAPKAP Kinase 3 Protein, unactive, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-586
50 µg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-586
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMAPKAP Kinase 3 Protein, unactive, 50 µg
OverviewN-terminal, GST-tagged, recombinant, human MAPKAP Kinase 3, amino acids 2-end
References
Product Information
Quality LevelMQ100
Applications
ApplicationUnactive, N-terminal, GST-tagged, recombinant, human MAPKAP Kinase 3, amino acids 2-end, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Application NotesActivation Assay
Biological Information
Sourceexpressed in E.coli cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a member of the Ser/Thr protein kinase family. This kinase functions as a mitogen-activated protein kinase (MAP kinase)- activated protein kinase. MAP kinases are also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This kinase was shown to be activated by growth inducers and stress stimulation of cells. In vitro studies demonstrated that ERK, p38 MAP kinase and Jun N-terminal kinase were all able to phosphorylate and activate this kinase, which suggested the role of this kinase as an integrative element of signaling in both mitogen and stress responses. This kinase was reported to interact with, phosphorylate and repress the activity of E47, which is a basic helix-loop-helix transcription factor known to be involved in the regulation of tissue-specific gene expression and cell differentiation.
Gene Symbol
  • MAPKAPK3
  • MAPKAP
  • MAPKAP3
  • 3PK
  • MAPKAPK-3
  • 3pK
Protein TargetMAPKAP-K3
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q16644 # Modulator of polycomb-mediated repression, which can be activated either by ERK, p38 and JNK. Substrate of CSBP. In vitro, phosphorylates HSPB1, BMI1/PCGF4 and TCF3.
SIZE: 382 amino acids; 42987 Da
SUBUNIT: Interacts with TCF3 and with polycomb proteins, such as PCH2 and BMI1/PCGF4.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Predominantly located in the nucleus, when activated it translocates to the cytoplasm.
TISSUE SPECIFICITY: Widely expressed, with a higher expression level observed in heart and skeletal muscle. No expression in brain.
SIMILARITY: SwissProt: Q16644 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight~69.8kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated to activate SAPK2a (14-587).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-586 04053252276767