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48-691MAG MILLIPLEX® Human Cell Health Magnetic Bead Panel

The Cell Health Panel is a valuable tool for researchers to assess changes of specific protein expression and phosphorylation levels in applications involving: drug toxicity screening, studying changes in cell health due to disease, or studying various cell death and survival pathways.

48-691MAG
96-well plate  96-well plate
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number48-691MAG
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Human Cell Health Magnetic Bead Panel
OverviewCell health is affected by the careful balance of various physiological processes and signaling pathways in response to diverse stimuli including drug treatment, disease, culture conditions, and stress. For example, cells respond to different stressors by expressing stress-related proteins that may lead to the activation of survival pathways or initiation of cell death. Identifying markers of specific stressors (for example oxidative, heat shock, genotoxicity, ER stress, inflammation, et cetera), activated repair or death pathways (for example, apoptosis, autophagy, DNA repair/damage, et cetera), and affected cellular functions (mRNA synthesis, chromatin condensation, proliferation, OXPHOS, et cetera) may elucidate further details about ongoing pathological processes or pharmacological response mechanisms. Cell health panel includes all the key markers a researcher would need to analyze the health and quality of the cells.
References
Product Information
Detection methodLuminex xMAP
ConfigurationPremixed
Precision, %
  • Intra-assay:< 14
  • Inter-assay: < 22
Panel TypeCell Signaling
Quality LevelMQ200
Applications
Application The Cell Health Panel is a valuable tool for researchers to assess changes of specific protein expression and phosphorylation levels in applications involving: drug toxicity screening, studying changes in cell health due to disease, or studying various cell death and survival pathways.
Key Applications
  • Multiplexing
  • Cell health assessment
  • Cell signaling
Application Notes This is an overnight assay.
• Each assay requires 25 µL per well of diluted or neat stimulated cell lysate.
• This is a qualitative assay and provides insights into the expression levels of the mentioned analytes in response to stress/treatment or condition.
• The suggested working range of protein concentration for the assay is 1 to 25 μg of total protein/well (25 μL/well at 40 to 1000 μg/mL).
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Complex IV
  • HIF-1α
  • HSP70
  • PARP (Cleaved)
  • CHOP
  • Ki67
  • LC3B
  • Polyubiquitin K48-linkage
  • GRP78
  • phosphorylated eIF4B (Ser422)
  • NF-κB (Ser536)
  • Histone H2A.x (Ser139)
  • Histone H3 (Ser10)
  • p53 (Ser15)
  • RNA Pol II (Ser2)
  • Cyclin B1
Physicochemical Information
Standard Curve Range
  • N/A
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96-well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
48-691MAG 04065272078665

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