数量	产品目录编号	订购说明	数量 / 包装	公开价
			96-Well Plate

数量	产品目录编号	订购说明	数量 / 包装	公开价
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

PRCYTMAG40K-PX25 MilliporeMILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

MSDS
小册子
技术信息
Protocols

Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology

Recommended Products

概述

Replacement Information

重要规格表

Analytes Available	Species Reactivity	Key Applications	Detection Methods
sCD137 Eotaxin/CCL11 sFasL FGF-2 Fractalkine/CX3CL1 Granzyme A Granzyme B IL-1α IL-2 IL-4 IL-6 IL-16 IL-17A IL-17E/IL-25 IL-21 IL-22 IL-23 IL-28A IL-31 IL-33 IP-10/CXCL10 MIP-3α/CCL20 Perforin RANTES/CCL5 TNFβ/LTA	NhP	Mplex	Luminex xMAP

Description
Catalogue Number	PRCYTMAG40K-PX25
Trade Name	MILLIPLEX
Description	MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay
Overview	To identify specific cytokines involved in any inflammatory or immune response, it may be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include: • More flexible plate and plate washer options • Improved performance with turbid serum/plasma samples • Assay results equivalent to non-magnetic beads • Automated magnetic washing avoids many problems associated with vacuum filtration washing Therefore, the MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel 2 enables you to focus on the therapeutic potential of cytokines, as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, dramatically improve productivity.
Background Information	Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

References

Product Information
Components	Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized Sample Plate, 96 well format, 1 plate 2 Foil plate sealers Assay Buffer, 2 bottles, 30 mL Bead Diluent, 1 bottle, 3.5 mL Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL Premixed 24-plex Beads, 1 bottle, 3.5 mL RANTES beads, 1 vial
Detection method	Luminex xMAP
Configuration	Premixed
Precision, %	Inter-assay: <15 Intra-assay: <10
Panel Type	MAGNETIC Cytokines/Chemokines
Quality Level	MQ200

Applications
Application	Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.
Key Applications	Multiplexing
Application Notes	• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended. • This assay requires 25 µl of 1:2 diluted plasma or serum or 25 μL cell culture supernatant per well. • RANTES/CCL5 is for tissue culture samples only and cannot be combined with other analytes in this panel when measuring serum or plasma due to different dilution needs.

Biological Information
Specificity	Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity	Non-human Primate
Analytes Available	sCD137 Eotaxin/CCL11 sFasL FGF-2 Fractalkine/CX3CL1 Granzyme A Granzyme B IL-1α IL-2 IL-4 IL-6 IL-16 IL-17A IL-17E/IL-25 IL-21 IL-22 IL-23 IL-28A IL-31 IL-33 IP-10/CXCL10 MIP-3α/CCL20 Perforin RANTES/CCL5 TNFβ/LTA
Cross Reactivity	This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.

Physicochemical Information
Sensitivity	Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range	RANTES/CCL5 (tissue culture): 1 – 750 pg/mL IL-17A: 1 – 1,500 pg/mL Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL Granzyme B: 4 – 4,000 pg/mL MIP-3α/CCL20: 7 – 7,000 pg/mL sFasL: 8 – 8,000 pg/mL sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL IL-21, IL-31: 15 – 15,000 pg/mL FGF-2, IL-33: 20 – 20,000 pg/mL IL-23: 24 – 25,000 pg/mL IL-1α, IL-28A: 29 – 30,000 pg/mL IL-16: 73 – 75,000 pg/mL Granzyme A: 78 – 80,000 pg/mL Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL IL-4: 146 – 150,000 pg/mL IL-17E/IL-25: 293 – 300,000 pg/mL

Physicochemical Information

Sensitivity

Refer to kit protocol for sensitivities of individual biomarkers.

Standard Curve Range

RANTES/CCL5 (tissue culture): 1 – 750 pg/mL
IL-17A: 1 – 1,500 pg/mL
Eotaxin/CCL11, IL-6: 3 – 3,000 pg/mL
Granzyme B: 4 – 4,000 pg/mL
MIP-3α/CCL20: 7 – 7,000 pg/mL
sFasL: 8 – 8,000 pg/mL
sCD137, IL-2, IP-10/CXCL10: 10 – 10,000 pg/mL
IL-21, IL-31: 15 – 15,000 pg/mL
FGF-2, IL-33: 20 – 20,000 pg/mL
IL-23: 24 – 25,000 pg/mL
IL-1α, IL-28A: 29 – 30,000 pg/mL
IL-16: 73 – 75,000 pg/mL
Granzyme A: 78 – 80,000 pg/mL
Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA: 98 – 100,000 pg/mL
IL-4: 146 – 150,000 pg/mL
IL-17E/IL-25: 293 – 300,000 pg/mL

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Recommended storage for kit components is 2 - 8°C.

Packaging Information
Material Size	96-well plate

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
产品目录编号	GTIN
PRCYTMAG40K-PX25	04055977133028

Documentation

Protocols

Title
Protocol- PRCYT2MAG40K, PRCYTMAG40K-PX24, PRCYTMAG40K-PX25, PRCY2MG40PMX24BK, PRCY2MG40PMX25BK

MILLIPLEX MAP Non-Human Primate Cytokine/Chemokine Panel II - 25-Plex Premixed Immunology Multiplex Assay MSDS

职位
物料安全数据表 (MSDS)

小册子

标题
The Power of Biomarker Analysis

技术信息

标题
96 well Template 2 Up
96 well Template Single
Quality Control Range Sheet- PRCYT-108 and PRCYT-208
Quality Control Ranges: Milliplex Non-Human Primate Cytokine Magnetic Bead Panel II

种类

Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Premixed Kits

此产品已添加到您的购物车。