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14-916 PEK Protein, active, 10 µg

Active, N-terminal, 6His-tagged, recombinant human PEK, amino acids 536-end. For use in Kinase Assays.

PEK Protein, active, 10 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-916
10 µg  Also available in 250 µg size - call for pricing and availability and reference catalog number 14-916M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

特卖

概述

Replacement Information

特卖

This enzyme is part of KinaseProfiler™, a custom inhibitor selectivity discovery service.
Description
Catalogue Number14-916
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPEK Protein, active, 10 µg
OverviewN-terminal, 6His-tagged, recombinant human PEK, amino acids 536-end.
References
Product Information
Presentation50 mM Tris-HCl, pH 7.5, 0.3 M NaCl, 0.1 mM EGTA, 0.03 % Brij-35, 270 mM sucrose, 0.1% 2-mercaptoethanol, 1mM benzamidine, 200 μM PMSF
Quality LevelMQ100
Applications
ApplicationActive, N-terminal, 6His-tagged, recombinant human PEK, amino acids 536-end. For use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PEK expressed by baculovirus in Sf21 insect cells
Entrez Gene Number
Gene Symbol
  • PERK
  • WRS
  • EIF2AK3
  • DKFZp781H1925
Purification MethodImmobilized metal affinity chromatography (IMAC)
UniProt Number
UniProt SummaryFUNCTION: Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin D1 (By similarity).

SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

TISSUE SPECIFICITY: Ubiquitous. A high level expression is seen in secretory tissues.

PTM: Autophosphorylated. N-glycosylated (By similarity).

SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. Contains 1 protein kinase domain.
Molecular Weight71 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of RSRSRSRSRSRSRSR. For Specific Activity Data, refer to the Certificate of Analysis for individual lots of this enzyme.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size - call for pricing and availability and reference catalog number 14-916M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-916 04053252556296