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14-504M PKCδ Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKCδ, for use in Kinase Assays.

14-504M
250 µg  Also available in 10 μg size (cat#14-504) and in bulk (cat# 14-504-K).
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-504M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKCδ Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant amino acids 2-end, of human PKCδ

PKC Lipid activator (Catalog # 20-133) is required for use.
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant amino acids 2-end, of human PKCδ, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceHuman PKCδ expressed in Sf21 cells.
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummaryProtein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. Studies both in human and mice demonstrate that this kinase is involved in B cell signaling and in the regulation of growth, apoptosis, and differentiation of a variety of cell types. Alternatively spliced transcript variants encoding the same protein have been observed.
Gene Symbol
  • PRKCD
  • MGC49908
  • MAY1
  • nPKC-delta
Protein TargetPKCδ
Purification MethodNi2+/NTA-agarose
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05655 # This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function.
SIZE: 676 amino acids; 77477 Da
SUBUNIT: Interacts with PDK1 (By similarity). Interacts with RAD9A and CDCP1.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Membrane; Peripheral membrane protein (By similarity).
DOMAIN: SwissProt: Q05655 The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor. & The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
PTM: Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity (By similarity).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 C2 domain. & Contains 2 phorbol-ester/DAG-type zinc fingers. & Contains 1 protein kinase domain.
Molecular Weight78.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of PKC Substrate Peptide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-504) and in bulk (cat# 14-504-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-504M 04053252320019

Documentation

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-504 PKCδ Protein, active, 10 µg

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases