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HTS146RTA Ready-to-Assay™ mGlu2 Metabotropic Glutamate Receptor Frozen Cells

Human mGlu2 GPCR frozen cells for Calcium Flux FLIPR Assays.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Host Cell: Chem-1 Entrez Gene Number: NM_000839
HTS146RTA
1 kit  
Purchase on Sigma-Aldrich

概述

Replacement Information

重要规格表

SpeciesHost CellsProtein Target
Human Chem-1mGlu2
Description
Catalogue NumberHTS146RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ mGlu2 Metabotropic Glutamate Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Glutamate is a main excitatory neurotransmitter in the central nervous system, and it plays a role in learning, memory and neurotoxicity. The biological actions of glutamate are mediated by ionotropic and metabotropic glutamate receptors, which are ion channels and GPCRs respectively. Metabotropic glutamate receptors (mGluRs) are members of the class 3 G-protein coupled receptor family, which are characterized by a large extracellular domain. They are further classified into group I, II, and III mGluRs on the basis of their sequence identity, pharmacology, and signal transduction mechanism. Group I (mGlu1 and mGlu5) couple to the phospholipase C pathway through Gαq, whereas group II (mGlu2 and mGlu3) and group III (mGlu4, mGlu6, mGlu7, and mGlu8) negatively couple to the adenylyl cyclase pathway though Gαi (Conn and Pin, 1997). Agonists of the Group II metabotropic glutamate receptors, mGlu2 and mGlu3, display efficacy in animal models of anxiety and psychosis. A key role for mGlu2 in mediating these effects is indicated by the observation that selective allosteric potentiator of mGlu2 also retains antipsychotic-like activities in mice (Galici et al., 2005). In addition, mGlu2/3 agonists display analgesic activity in animal models (Jones et al., 2005). Millipore’s cloned human mGlu2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant mGlu2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at mGlu2.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman mGlu2 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassC
Host CellsChem-1
Protein TargetmGlu2
Target Sub-FamilyGlutamate (metabotropic)
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
HTS146RTA 04053252336935

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