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SAE178-100UG SAvPhire Monomeric Streptavidin iFluor™ 647

SAvPhire Monomeric Streptavidin iFluor 647, Cat. No. SAE178, is a high-affinity recombinant variant of SA expressed in E. coli that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules.

SAE178-100UG
100 μg  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue NumberSAE178-100UG
DescriptionSAvPhire Monomeric Streptavidin iFluor™ 647
Alternate Names
  • SAvPphire-iFluor™647
  • mSA-iFluor™647
Background InformationStreptavidin, a tetrameric protein, has been extensively used in purification and detection of biomolecules. The strong streptavidin-biotin bond can highly suited for attaching various biomolecules to one another or onto a solid support. The streptavidin-biotin complex displays high resistance to organic solvents, denaturants, detergents, and proteolytic enzymes. It can also withstand extremes of temperature and pH. However, one challenge faced by researchers using the streptavidin-biotin system is the target aggregation, which results from multivalent binding. It can cause cross-linking that can interfere with observations at the molecular level. These challenges can be overcome by use of monomeric streptavidin (mSA) that displays structural and binding properties that are suitable for multiple applications based on its small size and monovalent biotin binding. The monomeric form allows for association with biotinylated ligands without inducing aggregation that are observed with multivalent interactions. Monomeric form can also be genetically fused to another protein to form a biotin-binding tag. SAvPhire Monomeric Streptavidin is a is a high-affinity recombinant variant of streptavidin that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation steps with biotinylated molecules and offers easier access to the desired targets. Unlike other non-engineered monomeric forms, SAvPhire Monomeric Streptavidin displays highly desirable dissociation kinetics (koff = 63 x 10-3 min-1 for non-engineered monomeric form and 2.1 x 10-3 min-1 for SAvPhire Monomeric Streptavidin) and longer t1/2 (11 min vs 329 min). It is suitable for multiple immunodetection applications. (Ref.: Kroestch, A., et al., (2018). Appl. Microbiol. Biotechnol., 102(23); 10079-10089).
References
Product Information
FormatHRP
PresentationSAvPhire Monomeric Streptavidin Alpha Fluor™ 647 lyophilized from PBS containing D-Mannitol and Sucrose without azide.
Quality LevelMQ200
Applications
ApplicationSAvPhire Monomeric Streptavidin iFluor 647, Cat. No. SAE178, is a high-affinity recombinant variant of SA expressed in E. coli that minimizes the risk of aggregation, precipitation, and crosslinking in pre-incubation with biotinylated molecules.
Key Applications
  • Immunocytochemistry
  • Western Blotting
  • Flow Cytometry
Application NotesWestern Blotting Analysis: Mouse brain tissue lysate probed with a 1:1 mixture of Anti-GFAP-Biotin conjugate (1:1,000 dilution) and SAvPhire Monomeric Streptavidin iFluor 647 detected GFAP.

Flow Cytometry Analysis: CD4-Biotin was detected in untreated Human peripheral blood mononuclear cells (PBMC) stained with a premixed solution of Anti-CD-4 biotin, clone SK3 and Streptavidin iFluor 647.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
EpitopeUnknown
ConcentrationUpon reconstitution in 100 mL of distilled water the concentration will be 1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostE. coli
SpecificitySAvPhire Monomeric Streptavidin expressed in E. coli and conjugated with iFluor 647 that is suitable for immunodetection of biotinylated proteins.
Species Reactivity
  • All
Species Reactivity NotePredicted to react with all species.
Antibody TypeMonoclonal Antibody
UniProt Number
Molecular Weight15.5 kDa for SAvPhire Monomeric Streptavidin (with an N-terminal His-tag and a C-terminal FLAG® tag).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

Immunofluorescent analysis of A431 cells performed using a 1:1 mixture of biotinylated Anti-EGFR (1:1,000 dilution) and SAvPhire Monomeric Streptavidin iFluor 647 detected EGFR.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
SAE178-100UG 04065265086066

Documentation

SAvPhire Monomeric Streptavidin iFluor™ 647 MSDS

职位

物料安全数据表 (MSDS) 

SAvPhire Monomeric Streptavidin iFluor™ 647 分析证书

标题批号
SAvPhire Monomeric Streptavidin iFluor™ 647 - 4060812 4060812
SAvPhire Monomeric Streptavidin iFluor™ 647 - 4197793 4197793
SAvPhire Monomeric Streptavidin iFluor™ 647 - 4200093 4200093
SAvPhire Monomeric Streptavidin iFluor™ 647 - 4248901 4248901
SAvPhire Monomeric Streptavidin iFluor™ 647 - 4273739 4273739
SAvPhire Monomeric Streptavidin iFluor™ 647 - Q3528201 Q3528201