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17-285 TCF Reporter Plasmid Kit

Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.

TCF Reporter Plasmid Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
17-285
1 kit  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number17-285
Brand Family Upstate
Trade Name
  • Upstate
DescriptionTCF Reporter Plasmid Kit
OverviewFOPflash: Transfection grade T cell factor (TCF) reporter plasmid containing two full and one incomplete copy of the TCF binding site (mutated) followed by three copies in the reverse orientation, upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. This plasmid serves as a negative control to TOPflash which contains wild type TCF binding sites.

TOPflash: Transfection grade T-cell factor (TCF) reporter plasmid containing two sets (with the second set in the reverse orientation) of three copies of the TCF binding site (wild type) upstream of the Thymidine Kinase (TK) minimal promoter and Luciferase open reading frame. FOPflash containing mutated TCF binding sites is also available as a negative control.
Alternate Names
  • TOPFlash/FOPFlash
  • TOPFlash plasmid
  • FOPFlash plasmid
Background InformationThe Wnt family encodes secreted glycoproteins implicated in cell growth regulation, cell fate determination, organogenesis and oncogenesis. Inferred from genetic analysis of wingless (wg) signaling in Drosophila, in mammalian cells, Wnts signal through the Frizzled family of receptors, to Dishevelled. Dishevelled down regulates glycogen synthase kinase-3ß (GSK-3ß) activity. This results in the stabilization and accumulation of cytosolic ß-catenin, a GSK-3ß substrate, which when phosphorylated by GSK-3ß, is targeted for ubiquitin-mediated proteolysis. ß-catenin then translocates to the nucleus, where in complexes with members of the Tcf/LEF family of transcription regulators, activates transcription of Tcf-responsive genes. This Tcf-reporter plasmid, along with FOPFLASH (Cat. # 21-169) enables quantitation of Wnt/Wg signaling in cells transfected with these constructs.
References
Product Information
Components
  • FOPflash (mutant TCF binding sites) (Cat.# 21-169)
  • TOPflash (TCF Reporter Plasmid) (Cat.# 21-170)
HS Code2934 99 99
PresentationFOPflash, Catalog # 21-169, Lot # 30860, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution.

TOPflash, Catalog # 21-170, Lot # 27752, 5.5 kb. 5 μg affinity purified DNA eluted and aseptically packaged in 50 μL of 10 mM Tris-HCl, pH 8.0, 1 mM EDTA. Frozen solution.
Quality LevelMQ100
Applications
ApplicationSet of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays.
Key Applications
  • Transfection
Biological Information
Entrez Gene Number
Entrez Gene SummaryThis gene encodes transcription factor 4, a basic helix-turn-helix transcription factor. The encoded protein recognizes an Ephrussi-box ('E-box') binding site ('CANNTG') - a motif first identified in immunoglobulin enhancers. This gene is expressed predominantly in pre-B-cells, although it is found in other tissues as well. Multiple alternatively spliced transcript variants that encode different proteins have been described.
Gene Symbol
  • TCF1
  • MODY3
  • HNF1
  • HNF-1A
  • TCF-1
  • HNF1A
  • LFB1
  • HNF1a
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P20823 # Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
SIZE: 631 amino acids; 67356 Da
SUBUNIT: Binds DNA as a dimer.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Liver.
DISEASE: SwissProt: P20823 # Defects in HNF1A may predispose to hepatic adenomas [MIM:142330]. Hepatic adenomas are benign tumors at risk of malignant transformation. Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas. & Defects in HNF1A are the cause of maturity onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY [MIM:606391] is a form of diabetes characterized by an autosomal dominant mode of inheritance, age of onset of 25 years or younger and a primary defect in insulin secretion. The clinical phenotype of MODY3 is characterized by severe insulin secretory defects, and by major hyperglycemia associated with microvascular complications. & Defects in HNF1A are a cause of susceptibility to insulin-dependent diabetes mellitus (IDDM) [MIM:222100].
SIMILARITY: SwissProt: P20823 ## Belongs to the HNF1 homeobox family. & Contains 1 homeobox DNA-binding domain.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by DNA Sequence Analysis to confirm the identity of TCF binding sites.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 2 years at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vials after thawing and prior to removing the cap.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
17-285 04053252516030

Documentation

TCF Reporter Plasmid Kit 分析证书

标题批号
TCF Reporter Plasmid Kit 2943905
TCF Reporter Plasmid Kit 3023379
TCF Reporter Plasmid Kit 2893733
TCF Reporter Plasmid Kit 3104977
TCF Reporter Plasmid Kit 3003553
TCF Reporter Plasmid Kit - 2433529 2433529
TCF Reporter Plasmid Kit - 0609040122 0609040122
TCF Reporter Plasmid Kit - 0703055812 0703055812
TCF Reporter Plasmid Kit - 19030 19030
TCF Reporter Plasmid Kit - 19495 19495
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参考

参考概述公共医疗ID
Selective small molecule inhibitors of glycogen synthase kinase-3 modulate glycogen metabolism and gene transcription
Coghlan, M P, et al
Chem Biol, 7:793-803 (2000)  2000

11033082 11033082
The TAK1-NLK-MAPK-related pathway antagonizes signalling between beta-catenin and transcription factor TCF.
Ishitani, T, et al.
Nature, 399: 798-802 (1999)  1999

显示摘要 		10391247 10391247
Tcf-1-mediated transcription in T lymphocytes: differential role for glycogen synthase kinase-3 in fibroblasts and T cells
Staal, F. J., et al
Int Immunol, 11:317-23 (1999)  1999

10221643 10221643
The Xenopus Wnt effector XTcf-3 interacts with Groucho-related transcriptional repressors.
Roose, J, et al.
Nature, 395: 608-12 (1998)  1998

显示摘要 		9783587 9783587
Two members of the Tcf family implicated in Wnt/beta-catenin signaling during embryogenesis in the mouse.
Korinek, V, et al.
Mol. Cell. Biol., 18: 1248-56 (1998)  1998

显示摘要 		9488439 9488439
Armadillo coactivates transcription driven by the product of the Drosophila segment polarity gene dTCF
van de Wetering, M, et al
Cell, 88:789-99 (1997)  1997

9118222 9118222