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2140 Total Protein Extraction Kit

Total Protein Extraction Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Protein Extraction
2140
1 kit  
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number2140
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionTotal Protein Extraction Kit
OverviewTotal protein extraction kit is an excellent tool for the initial purification and preparation of proteins from any tissues and cells. Total protein isolated by this kit is native and can be used for downstream applications including SDS-PAGE, Western blotting, gel mobility shift, protein assays and other procedures. The kit is easy to use: no scraping, no freeze-thaw cycles, and no sonication.

This kit contains enough reagents for the isolation of protein from 5 grams of tissue or cultured cells.
Materials Required but Not Delivered· Tissue Homogenizer

· Centrifuge and Microcentrifuge

· Rolling facility
References
Product Information
Components
  • TM Buffer 13 mL (Stored at 2-8°C)
  • 50X PI Buffer 260 μL (Stored at -20°C)
Applications
Key Applications
  • Protein Extraction
Application Notes· Isolate protein from large samples

· Isolate protein either from tissues or from cultured cells

· Protein is intact, and some proteins purified by this kit could be over 200 kd in mass

The isolated protein can be used for Western Blot, DNA-protein interaction, enzymatic activity analysis, protein-protein interaction, and immunoprecipitation and tissue specific expression identification.

PROTOCOL:

Important:

Aliquot 50X PI on ice and store at -20°C.

Always make fresh working solutions before the isolation of proteins.

1. Dilute 50X PI solution to 1X PI in TM buffer keeping the solution on ice.

2. Weigh certain tissues and chop them into small pieces.

3. Keep the tissues on dry ice.

4. Add 1X PI to the tissue at 2.5 mL per gram of tissue or per 25 million cells, and put in ice for 5 minutes.

5. Homogenize the tissue or cells for 20 seconds and then put on dry ice for 15 seconds.

6. Homogenize the tissue or cells for the second 20 seconds. (A third 20 seconds homogenization may be required if the tissue or cells are not well homogenized.)

7. Rotate the homogenized tissue or cells at 4°C for 20 minutes.

8. Centrifuge at 11,000 rpm at 4°C for 20 minutes.

9. Collect the supernatant.

10. Determine the concentration of the total protein.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore the TM Buffer at 2-8°C for up to 12 months after date of receipt. Store the 50X PI Buffer at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
2140 04053252004391

Documentation

Total Protein Extraction Kit MSDS

职位

物料安全数据表 (MSDS) 

Total Protein Extraction Kit 分析证书

标题批号
TOTAL PROTEIN EXTRACTION KIT VP1809143
TOTAL PROTEIN EXTRACTION KIT VP1803160
TOTAL PROTEIN EXTRACTION KIT 2856132
TOTAL PROTEIN EXTRACTION KIT 2944808
TOTAL PROTEIN EXTRACTION KIT 2925324
TOTAL PROTEIN EXTRACTION KIT 2475625
TOTAL PROTEIN EXTRACTION KIT 2901861
TOTAL PROTEIN EXTRACTION KIT - 2135094 2135094
TOTAL PROTEIN EXTRACTION KIT - 2390373 2390373
TOTAL PROTEIN EXTRACTION KIT - 1975920 1975920
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