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14-772 ULK2, active

ULK2, active MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
14-772
10 µg  Also available in 250μg size as catalog # 14-772M and in bulk as catalog # 14-772-K.
Purchase on Sigma-Aldrich

概述

Replacement Information
Description
Catalogue Number14-772
Trade Name
  • Upstate
DescriptionULK2, active
OverviewSerine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of
mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may
govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons
References
Product Information
PresentationProtein in 50mM Tris/HCl pH7.2, 300mM NaCl, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.03% Brij-35. Frozen solution.
Quality LevelMQ100
Applications
Key Applications
  • Kinase Assay
Biological Information
SourceN-terminal GST and C-terminal 6His-tagged, recombinant, human, ULK2, amino acids 1–306. Expressed by baculovirus in Sf21 insect cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Gene Symbol
  • ULK2
Purification MethodPurified using glutathione agarose.
UniProt Number
Molecular Weight62 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of casein
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250μg size as catalog # 14-772M and in bulk as catalog # 14-772-K.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
14-772 04053252282676

相关产品和应用

Alternative Packsize

产品目录编号 描述
14-772M ULK2, active

产品族

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种类

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases