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CBL202-K Vimentin Ms x Hu Mg

This Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.

CBL202-K
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概述

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Description
Catalogue NumberCBL202-K
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionVimentin Ms x Hu Mg
OverviewThis product is intended for use by IVD manufacturers.
Contact customer service for assistance: oem.reagents@emdmillipore.com
Alternate Names
  • Ant- Vimentin
References
Product Information
FormatPurified
Control
  • Positive in RD cells, glioma cells, fibroblasts (SV-80), and MDCK cell line
PresentationProtein A Purified immunoglobulin presented as lyophilized material. Reconstitute with 1ml distilled water. Final solution is PBS, pH 7.4, containing 0.5% BSA and 0.09% sodium azide.
Quality LevelMQ300
Applications
ApplicationThis Anti-Vimentin Antibody, clone VIM 3B4 is validated for use in ELISA, IF, IH(P), WB for the detection of Vimentin.
Key Applications
  • ELISA
  • Immunofluorescence
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesWestern blot
Immunohistochemistry: 1:2000; Frozen and paraffin-embedded tissue sections with a minimum of a one hour incubation (longer for paraffin); protease pretreatment is recommended for paraffin-embedded sections.
Immunofluorescence
ELISA

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenVimentin purified from bovine lens
CloneVIM 3B4
Concentration0.05 mg/mL
HostMouse
SpecificityHighly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This antibody is positive with cells of mesenchymal derivation, including endothelial cells and certain smooth muscle cells of the vascular tract, fibroblasts, connective tissue, all types of blood cells, including thymocytes, interstitial and testicular Sertoli cells, ovarian follicle cells. It can also be used to detect coexpression of vimentin in combination with other intermediate filament proteins. VIM 3B4 has turned out to be the most avid monoclonal antibody to vimentin. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995). Polypeptide reacting: MW 57 kDa intermediate filament protein (vimentin) of mesenchymal cells.
IsotypeIgG2a
Species Reactivity
  • Amphibian
  • Bovine
  • Canine
  • Chicken
  • Human
  • Monkey
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryAlong with the microfilaments (actins) and microtubules (tubulins), the intermediate filaments represent a third class of well-characterized cytoskeletal elements. The subunits display a tissue-specific pattern of expression. Desmin (MIM 125660) is the subunit specific for muscle and vimentin the subunit specific for mesenchymal tissue.[supplied by OMIM]
Gene Symbol
  • VIM
  • FLJ36605
  • Vimentin.
  • vimentin
Purification MethodProtein A Purfied
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08670 # Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
SIZE: 466 amino acids; 53652 Da
SUBUNIT: Homopolymer. Component of a complex composed at least of ACTB, AP2M1, AP2A1, AP2A2, MEGF10 and VIM. Interacts with HCV core protein.
TISSUE SPECIFICITY: Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines.
PTM: One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized.
SIMILARITY: SwissProt: P08670 ## Belongs to the intermediate filament family.
Molecular Weight57 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2–8°C for up to 12 months from date of receipt.
Packaging Information
Material SizeBulk packaging. Please inquire.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
产品目录编号 GTIN
CBL202-K 04053252477430

Documentation

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产品目录编号 描述
CBL202 Anti-Vimentin Antibody, clone VIM 3B4

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