Chromolith® SemiPrep HPLC Columns

Request Information

Perfect Scale-Up from Analytical to Preparative LC

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/LE-Chrom-Chromolith SemiPrep-HPLC column-150x724-20140214.png

Chromolith® SemiPrep HPLC columns are ideally suited for direct scale-up from analytical to semi-prep. They offer faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles. The columns are also excellent for solving separation problems with non-polar basic and acidic compounds, as well as peptides.

With an internal diameter (i.d.) of 10 mm, Chromolith® SemiPrep columns are the perfect alternative to particulate columns of 10 mm i.d. (and even 21.2 mm i.d). Furthermore, the selectivity of Chromolith® RP-18 endcapped SemiPrep columns is comparable to common RP-18 endcapped reversed phase columns.

Bimodal Porous Silica Rod Structure

The columns also offer an extended lifetime thanks to their rugged monolithic silica structure, which is the same as that of Chromolith® analytical columns with an internal diameter of 4.6 mm. The 2 μm macropores of the silica rod dramatically reduce separation time, while the 13 nm mesopores create a large surface area for adsorption of target molecules.

Specifications

Features and Benefits

  • Direct scale-up from analytical to semi-prep
  • Faster sample throughput at a lower operating pressure compared to semi-prep columns packed with 5 μm particles
  • Sharp separations, even at high sample loading
  • Excellent column lifetime thanks to the rugged monolithic silica structure
  • Optimized for LC/MS by a surface modification process minimizing column bleeding

Optimum Separation at Various Flow Rates

Chromolith® SemiPrep RP-18 endcapped 100-10 mm i.d. columns combine high separation speed with excellent performance. They are the ideal alternative to particulate columns with 10 and 21.2 mm i.d.

Separation of a standard mixture

Sample Loadability

Chromolith® SemiPrep HPLC columns deliver sharp separations, even at high sample loading.

Separation of 80 mg/injection

Up-Scaling

A Chromolith® SemiPrep RP-18 endcapped column injected with a 25 mg sample demonstrates the same excellent separation properties as a corresponding analytical column.

Up-Scaling

 
 
 
色谱
应用说明查找器
按照分析物、色谱柱类型、检测方法、吸附剂类型或任何其他搜索项搜索色谱应用:
 
HPLC工作流程解决方案 — 固定相

HPLC工作流程解决方案

固定相

详细了解

分析应用领域

色谱应用领域

The ultimate source for the latest news in your field

查看新闻
Chromolith® WidePore 300 HPLC Columns

Video

Chromolith® WidePore 300 HPLC Columns

Watch Video
 

大事记

十一月 10
11/10/20 - 11/12/20
BrauBeviale
Nürnberg Exhibition Centre
Nuremberg, Germany
水的提纯,试剂、化学制剂及实验室用品,工业微生物,分析及样品的制备 欧洲 20201110