Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot.
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions.
... and pathway assays, with relevant lysate controls included in each kit, these lysates are also recommended for use in Western blotting and immunoprecipitation. Browse our MILLIPLEX® Cell Signaling Lysates Lysate and Treatment Catalogue Number HeLa: ...
Mesenchymal stem cell lines are defined as adherent multipotent progenitor cells with the capacity to differentiate into several mesenchymal cell lineages.
Traditional protein assays are not suitable for analyzing extracellular vesicle preparations because the presence of lipids and other nonprotein components in the sample may interfere with protein analysis. Learn more about how IR-based assay-free quantitation afforded by Direct Detect system can be used to in extracellular vesicle preparation.
LC3-II enrichment Kit (Western Blot) enables quantification of autophagosome density by utilizing a permeabilization procedure that reveals quantities of LC3-II, without interference from LC3-I, by Western blotting analysis.
Extracellular matrix (ECM) proteins regulate cell adhesion, differentiation, proliferation, migration, invasion, and survival. The nature of neural architecture requires an intricate relationship with the ECM.
Our membrane protein extraction kits allow isolation of proteins from mammalian cells and tissue, and can enable extraction of transmembrane proteins such as GPCRs.