Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
All RIPAb+ antibodies are validated for RNA-binding protein immunoprecipitation. Each RIPAb+ antibody set includes control primers (each lot tested by qPCR) to biologically validate your RNA RIP results by qPCR detection of specific RNAs where possible
RNA-binding proteins (RBPs) play a key role in post-transcriptional regulation of gene expression by binding to RNA along various points and at various times.
RNA-binding protein immunoprecipitation (RIP) is the RNA analog of the ChIP application (chromatin immunoprecipitation). It can be used to identify specific RNA molecules (of many types) associated with specific nuclear or cytoplasmic binding proteins.
RIP kits Selection Guide gives more information and details about each type of RIP Kits: Description, Number of assays, Assay time, Availability with protein A or G, Agarose or Magnetic, etc..
Transcription factors are frequently the chief determinants of the composition and stability of large transcription complexes. Transcriptional regulation is mediated through the interactions of transcription factors with specific binding sites. Transcription factors help to recruit RNA polymerases to active genes for the production of RNA transcripts. Detect Transcription factors using Merck's antibodies.
The possible causes and potential remedies for challenges encountered in the immunoprecipitation-Western blot technique, which consists of cell lysis, formation of the antibody-antigen (immune) complex, precipitation of the immune complexes, and analysis by Western blotting.