OP15
Anti-c-ErbB2/c-Neu (Ab-3) Mouse mAb (3B5)
liquid, clone 3B5, Calbiochem®
동의어(들):
Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
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크기 선택
보기 변경
제품정보 (DICE 배송 시 비용 별도)
NACRES:
NA.43
UNSPSC Code:
12352203
Clone:
3B5, monoclonal
Species reactivity:
human, mouse
Application:
—
Citations:
38
biological source
mouse
Quality Segment
antibody form
purified antibody
antibody product type
primary antibodies
clone
3B5, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (2.5 µg/mL)
Immunoblotting (2-3 µg/mL,
Immunofluorescence (2.5 µg/mL)
Immunoprecipitation (1 µg/sample,
Paraffin Sections (2.5 µg/mL, pressure cooker, heat or trypsin pre-treatment required, )
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ERBB2(2064)
General description
Anti-c-ErbB2/c-Neu (Ab-3), mouse monoclonal, clone 3B5, recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells. It is validated for WB, IF, IP, and IHC on frozen and paraffin sections.
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0.Ag14 cells (see application references). Recognizes the ~185 kDa c-Erb2/c-Neu protein.
Recognizes the ~185 kDa c-ErbB2/c-Neu protein in HEK293 cells.
Immunogen
Human
a synthetic peptide (TAENPEYLGLDVPV) corresponding to amino acids 1242-1255 from the C-terminal domain of human c-ErbB2/c-Neu
Application
Frozen Sections (2.5 g/ml)
Immunoblotting (2-3 g/ml, see application references)
Immunofluorescence (2.5 g/ml)
Immunoprecipitation (1 g/sample, see application references)
Paraffin Sections (2.5 g/ml, pressure cooker, heat or trypsin pre-treatment required, see application references)
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Analysis Note
Negative Control
HepG2 cells or normal skin
HepG2 cells or normal skin
Positive Control
SK-BR-3 cells or breast carcinoma tissue
SK-BR-3 cells or breast carcinoma tissue
Other Notes
For paraffin sections, we recommend pre-treating with a pressure cooker, but trypsin and heat will also work well. Including 0.05% saponin during staining of paraffin sections may help reduce background. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribriform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.
Mamcs, J.R., et al. 1994. Ann. Surg.219, 332.
Tetu, B., et al. 1994. Cancer73, 2359.
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Bargmann, C.I., et al. 1986. Nature319, 226.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Tetu, B., et al. 1994. Cancer73, 2359.
DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Bargmann, C.I., et al. 1986. Nature319, 226.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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저장 등급
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.