C5233
Carboxypeptidase B from human pancreas
50-55 units/mg protein carboxypeptidase B
Sinónimos:
Peptidyl-L-Lysine[L-arginine] hydrolase
Iniciar sesión para ver los precios por organización y contrato.
Seleccione un Tamaño
Cambiar Vistas
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarlebiological source
human pancreas
Quality Level
form
solution
specific activity
50-55 units/mg protein carboxypeptidase B
impurities
≤0.2% chymotrypsin, ≤0.2% trypsin, ≤1 unit/mg protein carboxypeptidase A
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
SMILES string
S(Cc3ccccc3)C(=O)C(NC(=O)C2N(CCC2)C(=O)C(NC(=O)C(NC(=O)CCC(=O)O)C)C)Cc1ccccc1
InChI
1S/C31H38N4O7S/c1-20(32-26(36)15-16-27(37)38)28(39)33-21(2)30(41)35-17-9-14-25(35)29(40)34-24(18-22-10-5-3-6-11-22)31(42)43-19-23-12-7-4-8-13-23/h3-8,10-13,20-21,24-25H,9,14-19H2,1-2H3,(H,32,36)(H,33,39)(H,34,40)(H,37,38)
InChI key
TWURVFFNODFJBJ-UHFFFAOYSA-N
Gene Information
human ... CPB1(1360)
General description
Carboxypeptidase B is mapped to human chromosome 3q24. Carboxypeptidase B belongs to A/B subfamily of carboxypeptidases.
Application
Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.
Biochem/physiol Actions
Carboxypeptidase B (or peptidyl-L-lysine (-L-arginine) hydrolase) catalyzes the hydrolysis of the basic amino acids, lysine, arginine, and ornithine from the C-terminal position of polypeptides. It has been shown to be a single polypeptide of 34,000 Da. Trypsin is capable of converting native enzyme to the active enzyme, carboxypeptidase B II in vitro. The optimum pH is found to be 9.0. The enzyme may be used for sequence analysis by successive cleavage of C-terminal basic amino acids. It can also be used as a serum marker for the diagnosis of acute pancreatitis.
Mutations in the carboxypeptidase B (CPB1) gene is implicated with increased susceptibility to pancreatic cancer development and progression. Elevated levels of CPB1 is associated with low grade breast tumors and lymph node positive grade 1 tumors.
Physical form
Solution in 0.05 M NaOAc pH 5.0 + 1.0 M NaCl + 0.01% NaN3
Other Notes
One unit will hydrolyze 1 μmole of hippuryl-L-arginine per minute at pH 7.7 at 25 °C
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 1
Elija entre una de las versiones más recientes:
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Contenido relacionado
QC Methods
E Sim et al.
The Biochemical journal, 239(3), 763-767 (1986-11-01)
The plasma complement protein C4 is encoded at two highly polymorphic loci, A and B, within the class-III region of the major histocompatibility complex. At least 34 different polymorphic variants of human C4 have been identified, including non-expressed or 'null'
Xiu Qin Xu et al.
The Journal of biological chemistry, 280(25), 23987-24003 (2005-04-23)
We have exploited a discrepancy in the oncogenic potential of autocrine and exogenous human growth hormone (hGH) in an attempt to identify molecules that could potentially be involved in oncogenic transformation of the human mammary epithelial cell. Microarray analysis of
Lawrence L K Leung et al.
Advances in experimental medicine and biology, 632, 61-69 (2008-11-26)
Thrombin-activatable procarboxypeptidase B (proCPB or thrombin-activatable fibrinolysis inhibitor or TAFI) is a plasma procarboxypeptidase that is activated by the thrombin-thrombomodulin complex on the vascular endothelial surface. The activated CPB removes the newly exposed carboxyl terminal lysines in the partially digested