Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Yield effects on 2-methoxy-3-isobutylpyrazine concentration in cabernet sauvignon using a solid phase microextraction gas chromatography/mass spectrometry method.

Journal of agricultural and food chemistry (2004-08-19)

Dawn M Chapman, John H Thorngate, Mark A Matthews, Jean-Xavier Guinard, Susan E Ebeler

RESUMEN

A rapid and automated solid phase microextraction (SPME) stable isotope dilution gas chromatography/mass spectrometry (GC-MS) method for 2-methoxy-3-isobutylpyrazine (MIBP) quantification in red wine was developed. Wines with 30% (w/v) NaCl and 2-methoxy-(2)H(3)-3-isobutylpyrazine internal standard were sampled with a 2 cm divinylbenzene/carboxen/poly(dimethylsiloxane) SPME fiber for 30 min at 40 degrees C and analyzed by GC-MS. The method was used to measure MIBP concentrations in Cabernet Sauvignon wines that were produced from six winter pruning treatments over two vintages. MIBP concentrations were significantly negatively correlated with buds per vine. In addition, the MIBP concentration was directly related to sensory vegetal intensity ratings obtained by descriptive analysis.

MATERIALES

Número de producto

Marca

Descripción del producto

57298-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, plain gray hub

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57328-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57299-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 23 ga, Autosampler, pk of 3, notched gray hub

57329-U

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Montaje de fibra SPME, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

20358

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ORBO^™ 90 Carboxen^® 564 (20/45) 160/80 mg, W,F,F separators, O.D. × L 6 mm × 75 mm, pkg of 25 ea, for analyte group MEK (methylethyl ketone)

20355

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ORBO-78 400/200 MG PK/25, W,W,W separators, O.D. × L 6 mm × 110 mm, pkg of 25 ea

25467

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Columna capilar para GC Carboxen^®-1010 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

10478-U

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57318

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25461

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Columna capilar para GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.53 mm, average thickness 30 μm

57319

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Fibra de carboxeno/polidimetilsiloxano (DVB/CAR/PDMS) de SPME, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, light blue hub

24246

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47528-U

Supelco

2-Isobutyl-3-methoxypyrazine solution, certified reference material

W313203

Sigma-Aldrich

2-Isobutil-3-metoxipirazina, ≥99%, FG

57335-U

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Fibra de carboxeno/polidimetilsiloxano (DVB/CAR/PDMS) de SPME, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

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297666

Sigma-Aldrich

2-Isobutil-3-metoxipirazina, 99%

24241-U

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Columna capilar para GC Carboxen^®-1006 PLOT, L × I.D. 30 m × 0.32 mm, average thickness 15 μm

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