Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Antagonistic and synergistic effects of light irradiation on the effects of copper on Chlamydomonas reinhardtii.

Aquatic toxicology (Amsterdam, Netherlands) (2014-07-30)

Giulia Cheloni, Claudia Cosio, Vera I Slaveykova

ABSTRACT

The present study showed the important role of light intensity and spectral composition on Cu uptake and effects on green alga Chlamydomonas reinhardtii. High-intenisty light (HL) increased cellular Cu concentrations, but mitigated the Cu-induced decrease in chlorophyll fluorescence, oxidative stress and lipid peroxidation at high Cu concentrations, indicating that Cu and HL interact in an antagonistic manner. HL up-regulated the transcription of genes involved in the antioxidant response in C. reinhardtii and thus reduced the oxidative stress upon exposure to Cu and HL. Combined exposure to Cu and UVBR resulted in an increase of cellular Cu contents and caused severe oxidative damage to the cells. The observed effects were higher than the sum of the effects corresponding to exposure to UVBR or Cu alone suggesting a synergistic interaction.

MATERIALS

Product Number

Brand

Product Description

GF29197480

Copper - O.F.H.C., tube, 1000mm, outside diameter 10mm, inside diameter 8mm, wall thickness 1.0mm, 99.95+%

GF59430304

Copper, mesh, 150x150mm, nominal aperture 0.14mm, thickness 0.25mm, wire diameter 0.115mm, 100x100 wires/inch, open area 30.3%, plain weave mesh

GF53474980

Copper, tube, 100mm, outside diameter 12.7mm, inside diameter 10.26mm, wall thickness 1.22mm, as drawn, 99.9%

GF07134960

Copper, insulated wire, 50m, conductor diameter 0.125mm, insulation thickness 0.01mm, polyimide insulation, 99.99%

GF93436926

Copper, tube, 1000mm, outside diameter 0.95mm, inside diameter 0.29mm, wall thickness 0.33mm, as drawn, 99.9%

GF29608904

Copper, insulated wire, 50m, conductor diameter 0.45mm, insulation thickness 0.25mm, PTFE (polytetrafluoroethylene) insulation

GF06390525

Copper - O.F.H.C., tube, 200mm, outside diameter 0.38mm, inside diameter 0.22mm, wall thickness 0.08mm, 99.95+%

GF01913419

Copper - O.F.H.C., foil, 0.5m coil, thickness 0.006mm, hard, 99.95+%

GF56507373

Copper - O.F.H.C., wire reel, 20m, diameter 2.0mm, hard, 99.95+%

GF51989248

Copper - O.F.H.C., foil, 300x300mm, thickness 0.25mm, half hard, 99.95+%

GF13846877

Copper, rod, 100mm, diameter 16mm, as drawn, 99.99+%

GF93351304

Copper, mesh, 150x150mm, nominal aperture 0.38mm, wire diameter 0.25mm, 40x40 wires/inch, open area 37%, plain weave mesh

GF01112724

Copper, insulated wire, 5m, conductor diameter 0.1mm, insulation thickness 0.015mm, 10-30 micron polyesterimide insulation, 99.9%

GF64661687

Copper - O.F.H.C., tube, 200mm, outside diameter 6.0mm, inside diameter 4mm, wall thickness 1.0mm, 99.95+%

GF51968908

Copper - O.F.H.C., wire reel, 50m, diameter 1.0mm, as drawn, 99.95+%

GF55859855

Copper, insulated wire, 2m, conductor diameter 1.0mm, insulation thickness 0.25mm, PTFE (polytetrafluoroethylene) insulation

GF92764974

Copper - O.F.H.C., foil, 50x50mm, thickness 0.1mm, hard, 99.95+%

GF56099571

Copper, insulated wire, 200m, conductor diameter 0.5mm, insulation thickness 0.06mm, polyimide insulation

GF00920898

Copper, rod, 100mm, diameter 9.5mm, hard, 99.9%

GF41407501

Copper, microfoil, disks, 10mm, thinness 0.25μm, specific density 219.9μg/cm², permanent mylar 3.5μm support, 99.99+%

GF55575647

Copper, insulated wire, 5m, conductor diameter 0.5mm, insulation thickness 0.025mm, polyimide insulation, 99.99%

GF69447280

Copper, tube, 100mm, outside diameter 0.75mm, inside diameter 0.23mm, wall thickness 0.26mm, as drawn, 99.9%

GF33693194

Copper, insulated wire, 50m, conductor diameter 0.5mm, insulation thickness 0.06mm, polyimide insulation

GF20236878

Copper, insulated wire, 20m, conductor diameter 0.45mm, insulation thickness 0.25mm, PTFE (polytetrafluoroethylene) insulation

GF16694006

Copper, rod, 50mm, diameter 6.35mm, as drawn, 99.999%

GF61058156

Copper, insulated wire, 10m, conductor diameter 0.5mm, insulation thickness 0.06mm, polyimide insulation

GF53876256

Copper, rod, 200mm, diameter 8.0mm, as drawn, 99.99+%

GF80261669

Copper, mesh, 260x260mm, nominal aperture 0.2mm, thickness 0.004mm, wire diameter 0.0125mm, 118 wires/inch, open area 88%, electro-formed mesh

GF70627529

Copper - O.F.H.C., sheet, 150x150mm, thickness 6.35mm, 99.95+%

GF14145689

Copper, mesh, 600x600mm, nominal aperture 0.38mm, wire diameter 0.25mm, 40x40 wires/inch, open area 37%, plain weave mesh