Purification of mAbs and related proteins:
Chromatographic methods, strategies and optimization
September 4-6, 2017
Course Description
This hands-on course provides a fundamental understanding of chromatographic methods and strategies to purify monoclonal antibodies and related molecules.
The combination of theoretical lectures, industrial case studies and experiments in the lab enables participants to gain a profound knowledge of biochromatography processes.
Venue
Steinbeis Transfer Center at Mannheim University of Applied Sciences
Paul-Wittsack-Str. 1068163 Mannheim, Germany
Email: stz-tb@hs-mannheim.de
Registration Info
Due to the hands-on nature of this course, enrollment is limited to 8 participants. We recommend early booking to ensure a place.
Course fee: 950,00 €
To register, please contact Mrs. Andrea Bentz, Assistant to Prof. Dr. C. Frech (Email: stz-tb@hs-mannheim.de).
Course Content
- Theory and application of Protein A, ion exchange and mixed-mode chromatography
- Purification strategies for antibodies and related molecules
- Case studies presented by experts from industry
- Quality and safety aspects
Learning Goals
- Designing robust chromatographic unit operations
- Defining and optimizing suitable purification strategies
- Evaluating the pros and cons of different techniques
- Selecting analytical tools for process development
- Understand and use all the tools needed to remove process impurities and contaminants
Who should attend?
- Process development scientists
- Chemical and biochemical engineers
- R&D scientists and technicians
- Professionals in areas related to antibody processing
What will you receive?
- A comprehensive course documentation
- Summary of experimental results
- Certificate of participation
Course Schedule and Program
Day 1: Start 12 am
- Registration and Welcome
- Basic principles of antibody purification
- Antibody structure
- Protein A affinity chromatography
- Antibody purification by ion exchange chromatography
- Static and dynamic binding capacities
- Practical part I
- Capture of antibodies by Protein A affinity chromatography
- Ion exchange chromatography for antibody purification
- Determination of dynamic IgG binding capacities
- Analysis and discussion of results
- Dinner
Day 2: 8.30 am - 5.30 pm
- Different chromatographic techniques for the purification of antibodies:
- Hydrophobic interaction chromatography, hydroxyapatite & mixed mode chromatography, size exclusion chromatography
- Case studies:
- Regulatory aspects in protein purification and impurities testing (Dr. Bettina Katterle)
- Practical part II
Design of an antibody purification process- Purification strategies and sequencing of purification steps
- Post-Protein-A – steps in antibody purification
- Optimization of intermediate purification steps
- Optimization of polishing steps
Day 3: 9 am - 16 pm
- Case studies:
- Production of monoclonal antibodies (Xavier Le Saoût)
- Scale up in chromatography
- Presentation and discussion of results
- ourse summary and final discussion
Course Instructors and Industry Experts
Prof. Dr. Christian Frech, Steinbeis Transfer Centre at the University of Applied Sciences, Mannheim
Short Biography:
Dr. Bettina Katterle, Head of Marketing & Sales, BSL BIOSERVICE Scientific Laboratories GmbH, Planegg, Germany
Short Biography:
Xavier Le Saoût, Associate Manager BioProcess Sciences, Merck KGaA, Darmstadt, Germany Healthcare, Corsier sur Vevey, Switzerland
Short Biography:
Dr. Lars H. Peeck, Senior Marketing Manager, Life Science, Merck KGaA, Darmstadt, Germany
Short Biography:
Dr. Lothar Jacob, Consultant, Darmstadt, Germany
Short Biography: