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Related Resources: Erenna® Brochure | SMCxPRO™ Platform Brochure | Analyte Quarterly The Erenna® Immunoassay system, originally developed by Singulex®, Inc., accurately, precisely and reliably quantifies low-abundance biomarkers at femtogram/mL concentrations. Ultrasensitive Single Molecule Counting (SMC™) technology enables better science by providing better sensitivity.
Scientists use the Erenna® Immunoassay System when they need:
Quantification of analyte concentration differences, especially at ultrasensitive levels.
Data for biomarkers that were previously undetectable or unquantifiable with traditional immunoassay formats.
Unparalleled precision and accuracy, with lower sample volumes and lower analyte concentrations.
A method to rapidly develop their own assays with their proprietary antibodies and targets.
An enthusiastic partner who will work with them to build assays to suit their discovery needs.
The Erenna® Immunoassay System includes fully integrated benchtop analytical instrumentation with dedicated software for automated data analysis.
< 100 DE
< 1 fM*
< 7% CV†
> 4 logs
*Determined from calibrator set: 0-300 fM of 150 kD antibody labeled with fluorophore †DE measurements from 30 fM calibrator, n=20
Microsoft Windows® 7 Operating Systems
A static IP address and an FTP server
Sgx link™ software included
Plate Format (Reading)
Instrument Dimensions and Weight
H: 400 mm (15.75 in)
W: 540 mm (21.25 in)
D: 575.6 mm (22.7 in)
W: 31.3 kg (69 lbs)
U.S.: 115 VAC, 50-60 Hz (operating range 90-125 V)
Int.: 230 VAC, 50-60 Hz (operating range 180-250 V)
Sgx link™ Software
Sgx link™ Software: Connecting Users with Data
All Erenna® Immunoassay Systems include proprietary software with a wizard-driven interface for simple operation and analysis of ultrasensitive Erenna® immunoassays. Sgx link™ software allows the user to be in control.
Achieve a 4-log+ linear dynamic range with the SMDCurveFit™ algorithm. This proprietary algorithm combines digital and analog data processing for analysis of baseline and elevated samples in the same run.
Step-by-step guide helps operators complete experiment setup and analysis.
Reusable templates save time and ensure consistent experimental setup.
Multiple Analysis Options
Different curve-fitting modes and outlier removal functions provide fine-tuning control of data analysis (see screenshot to the right).
Variable Data Acquisition Protocols
Read time may be selected (15s – 60s) to best meet your throughput needs and for fit-for-purpose biomarker measurements.
Data Export Options
Automatic generation of final report and multiple file export options help to organize and present data.
User Account and Data Management
Management tools are provided for you to add security and control account privileges to achieve GLP compliance.
SMC™ Immunoassay Kits & Reagents
SMC™ Immunoassay Kits using Single Molecule Counting (SMC™) technology (developed by Singulex, Inc.) enable you to reach the highest levels of sensitivity possible in an immunoassay format. SMC™ kits contain all reagents required to perform assays on the Erenna® Immunoassay System. These assays take advantage of a standard immunoassay workflow and may be configured with a proprietary paramagnetic microparticle solid phase capture for ultrasensitive biomarker quantification, or in a standard plate format when extreme sensitivity is not required.
Assay protocols are similar to existing sandwich ELISA methods with two key differences:
Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification.
SMC™ Immunoassay System detects analytes using Single Molecule Counting (SMC™) technology.