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Concentrate it, don't lose it. Cytochrome C (colored red) is concentrated by Amicon® filters (left), but it passes through centrifugal filters from other suppliers (right). |
Compare Amicon Ultra Filters to Other Centrifugal Filters
Amicon® Ultra filters concentrate your protein with higher protein recovery, for more accurate downstream results. In addition, the quality of materials and manufacturing mean that Amicon® Ultra filters leach fewer extractable impurities into your samples, which translates into higher signal-to-noise ratios for sensitive analyses.View Comparison Data
(Click each chart to enlarge.)| Higher protein recovery for more accurate downstream results: | Fewer extractable impurities for higher signal-to-noise ratios: |
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| Comparison of protein recovery from various centrifugal filters. The recovery of a 25 kDa protein (α-chymotrypsinogen A) solution (4 mL of 1 mg/mL in PBS, pH 7.4) was evaluated using two different 10 kDa centrifugal filters. Filters were centrifuged in a fixed angle rotor, at 7,500 x g, for 30, 20 and 15 minutes, respectively, as recommended by the manufacturers. It was expected that the 25 kDa protein would be retained by both 10 kDa filters. |
Comparison of extractable impurities from two centrifugal filters as detected by LC-UV. Samples of Milli-Q® water were spun for 30 minutes at 2,000 x g. Filtrate (1 mL) was taken for analysis by LC-UV. |