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123900 AKTide-2T

123900
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





An optimal peptide substrate for assaying Akt/PKB/Rac-protein kinase activity in vitro. The peptide undergoes phosphorylation at the Ser site (Km = 3.9 µM). Competitively inhibits histone H2B phosphorylation (Ki = 12 µM) by Akt.
Catalogue Number123900
Brand Family Calbiochem®
SynonymsARKRERTYSFGHHA
References
ReferencesObata, T., et al. 2000. J. Biol. Chem. 275, 36108.
Product Information
FormOff-white solid
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₇₄H₁₁₄N₂₈O₂₀
Chemical formulaC₇₄H₁₁₄N₂₈O₂₀
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥95% by HPLC
Physicochemical Information
Peptide SequenceH-Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

AKTide-2T Certificates of Analysis

TitleLot Number
123900

References

Přehled odkazů
Obata, T., et al. 2000. J. Biol. Chem. 275, 36108.

Brochure

Title
Akt
Biologics 31.1
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision09-April-2008 RFH
SynonymsARKRERTYSFGHHA
DescriptionAn optimal peptide substrate for assaying AKT/PKB/Rac-protein kinase activity in vitro. The peptide undergoes phosphorylation at the Ser site (Km = 3.9 µM). Reported to competitively inhibit histone H2B phospho-rylation (Ki = 12 µM) by AKT.
FormOff-white solid
FormulationSupplied as a trifluoroacetate salt.
Intert gas (Yes/No) Packaged under inert gas
Recommended reaction conditions
The reaction mixture (30 µl) contains 20 mM HEPES, pH 7.4, 100 µM ATP with 3 µCi of [γ-32P]-ATP, 10 mM DTT, 10 mM MgCl2, 0.5 mM EGTA, and 50 µM AKTide-2T. Reaction begins with the addition of 0.1 µg of purified AKT. Incubation is carried out at 25°C for designated time periods (5 to 15 min). Reaction is terminated by adding 10 µl of stop solution containing 8 N HCl and 1 mM ATP. Aliquots are then spotted on P81-phosphocellulose paper, washed four times in 0.5% phosphoric acid, and quantified by scintillation counting. Appropriate blanks are run concurrently.
Chemical formulaC₇₄H₁₁₄N₂₈O₂₀
Peptide SequenceH-Ala-Arg-Lys-Arg-Glu-Arg-Thr-Tyr-Ser-Phe-Gly-His-His-Ala-OH
Purity≥95% by HPLC
SolubilityDMSO (5 mg/ml) or H₂O (1 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesObata, T., et al. 2000. J. Biol. Chem. 275, 36108.