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164598
Sigma-AldrichAminopeptidase M, Porcine Kidney
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Description
Overview
Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
Catalogue Number
164598
Brand Family
Calbiochem®
Synonyms
Aminopeptidase M
References
References
Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.
Product Information
CAS number
1185-53-1, 7783-20-2, 7791-18-6, 9054-63-1
Activity
≥50 units/ml
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-<i>p</i>NA per min at 37°C, pH 7.2.
EC number
3.4.11.2
Form
White crystalline suspension
Formulation
Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
Aminopeptidase M, Porcine Kidney Certificates of Analysis
Title
Lot Number
164598
References
Přehled odkazů
Wachsmuth, E.E., et al. 1966. Biochemistry5, 169.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
05-April-2011 JSW
Synonyms
Aminopeptidase M
Description
Native amino peptidase M from porcine kidney. Metalloprotease that hydrolyzes N-terminal amino acids from almost all unsubstituted oligopeptides. Does not cleave X-Pro bonds or N-blocked amino acids. Useful in peptide sequencing. Inhibited by EDTA, bestatin, and amastatin.
Form
White crystalline suspension
Formulation
Crystalline suspension in 3.2 M (NH₄)₂SO₄, 10 mM MgCl₂, 10 mM Tris-HCl, pH 7.5.
Concentration Label
Please refer to vial label for lot-specific concentration
CAS number
1185-53-1, 7783-20-2, 7791-18-6, 9054-63-1
EC number
3.4.11.2
Contaminants
Oxidases: ≤3%
Specific activity
≥10 units/mg protein
Activity
≥50 units/ml
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of Leu-pNA per min at 37°C, pH 7.2.
Solubility
H₂O or physiological buffers
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 4 months at -20°C.