176871 Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells

176871
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      Přehled

      Replacement Information

      Tabulka spec. kláve

      Ceny a dostupnost

      Katalogové číslo DostupnostBalení ks/bal. Cena Množství
      176871-10UG
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      Dostupné omezené množství
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          Plastová ampulka 10 μg
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          Description
          OverviewRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. Inactivates bradykinin by the sequential removal of two C-terminal dipeptides. ACE is a type I membrane protein that exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular mass of the glycosylated protein appears larger than 120 kDa under either reducing or non-reducing condition.
          Catalogue Number176871
          Brand Family Calbiochem®
          SynonymsPeptidyl-Dipetidase A, ACE
          References
          ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
          Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
          Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
          Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
          Product Information
          Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
          FormLiquid
          FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
          Quality LevelMQ100
          Applications
          Biological Information
          Purity≥95% by SDS-PAGE
          Specific Activity≥1 unit/µg protein
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          ContaminantsEndotoxins: ≤1.0 EU/µg ACE
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells MSDS

          Title

          Safety Data Sheet (SDS) 

          Angiotensin Converting Enzyme, His•Tag®, Human, Recombinant, NSO Cells Certificates of Analysis

          TitleLot Number
          176871

          References

          Přehled odkazů
          Wakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
          Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
          Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
          Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-April-2008 RFH
          SynonymsPeptidyl-Dipetidase A, ACE
          DescriptionRecombinant, human ACE ectodomain (amino acids 30-1261) fused at the C-terminus to a His•Tag® sequence and expressed in NSO cells. ACE is a zinc metallopeptidase that cleaves the C-terminal dipeptide from angiotensin I to produce the potent vasopressor octapeptide angiotensin II. ACE gene polymorphism has been a recent topic in Alzheimer's disease studies. ACE is a type I membrane protein and exists in two isoforms, somatic and germinal. By SDS-PAGE, the apparent molecular weight of the glycosylated protein appears larger to be than 120 kDa under both reducing and non-reducing conditions.
          FormLiquid
          FormulationIn 75 mM NaCl, 12.5 mM Tris, 500 nM ZnCl₂, 40% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxins: ≤1.0 EU/µg ACE
          Specific activity≥1 unit/µg protein
          Unit definitionOne unit is defined as the amount of enzyme that will cleave 1 nmol MCA-RPPGFSAFK(DNP)-OH per min at 25°C, pH 6.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesWakutani, Y., et al. 2002. Ann. N. Y. Acad. Sci. 977, 232.
          Hu, J., et al. 2001. J. Biol. Chem. 276, 47863.
          Kessler, S.P., et al. 2000. J. Biol. Chem. 275, 26259.
          Soubrier, F. et al. 1998. Proc. Natl. Acad. Sci. USA. 85, 9386.