324405 Anti-Dopamine D₄ Receptor Rabbit pAb

324405
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      Přehled

      Replacement Information

      Tabulka spec. kláve

      Species ReactivityHostAntibody Type
      H, R Rb Polyclonal Antibody

      Ceny a dostupnost

      Katalogové číslo DostupnostBalení ks/bal. Cena Množství
      324405-100UL
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      Dostupné omezené množství
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          Contact Customer Service

          Plastová ampulka 100 ul
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          Description
          OverviewRecognizes the ~48-51 kDa dopamine D4 receptor in rat brain. Does not cross-react with other dopamine receptors.
          Catalogue Number324405
          Brand Family Calbiochem®
          References
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Product Information
          FormLyophilized
          FormulationUndiluted serum.
          Positive controlRat brain
          PreservativeNone
          Quality LevelMQ100
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesELISA (see comments)
          Immunoblotting (1:500)
          Immunohistochemistry (1:10,000)
          Application CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
          Biological Information
          Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Anti-Dopamine D₄ Receptor Rabbit pAb MSDS

          Title

          Safety Data Sheet (SDS) 

          Anti-Dopamine D₄ Receptor Rabbit pAb Certificates of Analysis

          TitleLot Number
          324405

          References

          Přehled odkazů
          Mignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision27-July-2007 RFH
          ApplicationELISA (see comments)
          Immunoblotting (1:500)
          Immunohistochemistry (1:10,000)
          DescriptionRabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~48-51 kDa D4 dopamine receptor.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic peptide corresponding to amino acids 176-185 of human dopamine D₄ receptor
          IsotypeIgG
          Specieshuman, rat
          Positive controlRat brain
          FormLyophilized
          FormulationUndiluted serum.
          PreservativeNone
          CommentsThis antibody has also been reported to work for ELISA. For blocking immunoblot membranes, use ONLY 4% goat serum or evaporated goat's milk. For immunohistochemistry, a 1:10,000 dilution of the reconstituted serum demonstrated specific staining of rat brain sections including olfactory bulb glomeruli, cortex layer 4, cortex layers 2 and 3 (dendrite staining), cortex layer 5 (apical dendrite staining), and the dentate gyrus of the hippocampus (diffuse staining in the molecular layer, punctuate staining in the granule cell layer).
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute the lyophilized antibody with 100 µl PBS containing 10 mg/ml BSA. For further dilution of the antiserum use a similar buffer formulation. Following reconstitution, refrigerate (4°C). Variables associated with assay conditions will dictate the proper working dilution.
          Toxicity Standard Handling
          ReferencesMignini, F., et al. 2000. J. Autonom. Pharmacol. 20, 325.