324620 Anti-EBP50 Rabbit pAb

324620
  
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      Přehled

      Replacement Information

      Tabulka spec. kláve

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~50 kDa EBP50 protein.
      Catalogue Number324620
      Brand Family Calbiochem®
      SynonymsAnti-Ezrin-radixin-moesin Binding Phosphoprotein
      Application Data
      Detection of mouse EBP50 by immunohistochemistry. Sample: Mouse airway tissue section. Primary antibody: Anti-EBP50 Rabbit pAb (Cat. No. 324620) (10 µg/ml). Detection: fluorescence.

      Detection of human EBP50 by immunoblotting. Sample: Recombinant human EBP50. Primary antibody: Anti-EBP50 Rabbit pAb (Cat. No. 324620) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesFouassier, L., et al. 2001. Hepatology 33, 166.2.
      Reczek, D., et al. 1997. J. Cell Biol. 139, 169.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Positive controlT84 or Calu3 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (0.5-1.5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml)
      Application CommentsAlso recognizes the recombinant human protein. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenrecombinant protein consisting human EBP50 fused to a His•Tag® sequence
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-EBP50 Rabbit pAb Certificates of Analysis

      TitleLot Number
      324620

      References

      Přehled odkazů
      Fouassier, L., et al. 2001. Hepatology 33, 166.2.
      Reczek, D., et al. 1997. J. Cell Biol. 139, 169.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-July-2007 RFH
      SynonymsAnti-Ezrin-radixin-moesin Binding Phosphoprotein
      ApplicationImmunoblotting (0.5-1.5 µg/ml)
      Immunoprecipitation (see comments)
      Paraffin Sections (10 µg/ml)
      Application Data
      Detection of mouse EBP50 by immunohistochemistry. Sample: Mouse airway tissue section. Primary antibody: Anti-EBP50 Rabbit pAb (Cat. No. 324620) (10 µg/ml). Detection: fluorescence.

      Detection of human EBP50 by immunoblotting. Sample: Recombinant human EBP50. Primary antibody: Anti-EBP50 Rabbit pAb (Cat. No. 324620) (1 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~50 kDa EBP50 protein.
      BackgroundEzrin-radixin-moesin (ERM) proteins provide structural linkages between integral plasma membrane proteins and the cortical actin cytoskeleton. The ~50 kDa ERM-binding protein (EPB50) also may be involved in the regulation of ion exchange. It consists of two PDZ domains and a C-terminal tail.
      HostRabbit
      Immunogen speciesHuman
      Immunogenrecombinant protein consisting human EBP50 fused to a His•Tag® sequence
      IsotypeIgG
      Specieshuman
      Positive controlT84 or Calu3 cells
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAlso recognizes the recombinant human protein. This antibody has also been reported to work for immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesFouassier, L., et al. 2001. Hepatology 33, 166.2.
      Reczek, D., et al. 1997. J. Cell Biol. 139, 169.