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178489 Apoptosis Inducer Set II

178489
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information
Description
Overview

This product has been discontinued.





This set contains six ready-to-use chemical reagents that induce apoptosis through different mechanisms. The set contains 100 µl DMSO solutions each of Aphidicolin (10 µg/µl; Cat. No. 178273); Betulinic Acid (20 µg/µl; Cat. No. 200498); Paclitaxel, Taxus brevifolia (2 µg/µl; Cat. No. 580555); Staurosporine, Streptomyces sp. (0.5 µg/µl; Cat. No. 569397); and 100 µl aqueous solutions of Doxorubicin, Hydrochloride (5 µg/µl, Cat. No. 324380); and Puromycin, Dihydrochloride (20 µg/µl; Cat. No. 540222). Recommended dilution for use: 1:1000. Supplied with a data sheet.
Catalogue Number178489
Brand Family Calbiochem®
References
ReferencesLing, Y., et al. 2001. ol Pharmacol. 59, 593.
Yang, X.H, et al. 2001. Cancer Res. 61, 348.
Sanwal, V., et al. 2001. Exp. Mol. Pathol. 70, 54.
Fulda, S., and Debatin, K.M. 2000. Med. Pediatr. Oncol. 35, 616.
Shim, Y.H., et al. 2000. Int. J. Hematol. 72, 484.
Sangfelt, O., et al. 1997. Cell Growth Differ. 8, 343.
Product Information
FormLiquid
Formulation100 µl each inhibitor in DMSO or H₂O
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38-41-45-46-60

Irritating to eyes, respiratory system and skin.
Risk of serious damage to eyes.
May cause cancer.
May cause heritable genetic damage.
May impair fertility.
S PhraseS: 26-36/37/39-45-53-28

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Avoid exposure - obtain special instructions before use.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Multiple Toxicity Values, refer to MSDS
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot, purge with inert gas, and freeze (-20°C). Once opened, stock solutions are stable for up to 3 months when stored under the indicated conditions.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

Apoptosis Inducer Set II Certificates of Analysis

TitleLot Number
178489

References

Přehled odkazů
Ling, Y., et al. 2001. ol Pharmacol. 59, 593.
Yang, X.H, et al. 2001. Cancer Res. 61, 348.
Sanwal, V., et al. 2001. Exp. Mol. Pathol. 70, 54.
Fulda, S., and Debatin, K.M. 2000. Med. Pediatr. Oncol. 35, 616.
Shim, Y.H., et al. 2000. Int. J. Hematol. 72, 484.
Sangfelt, O., et al. 1997. Cell Growth Differ. 8, 343.

Brochure

Title
Caspases and other Apoptosis Related Tools Brochure
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision26-August-2008 RFH
DescriptionContains 6 ready-to-use chemical reagents that induce apoptosis through different mechanisms. Aphidicolin, is a specific inhibitor of DNA polymerase α and δ. Betulinic Acid is an antitumor and anti-HIV agent. Paclitaxel, Taxus brevifolia is an antitumor and antileukemic agent. Staurosporine, Streptomyces sp. is a potent, cell-permeable, and broad spectrum inhibitor of protein kinases that also induces apoptosis. Doxorubicin, Hydrochloride is an antitumor antibiotic and a highly effective myotoxin that inhibits topoisomerase II. Puromycin, Dihydrochloride is a nucleotide that resembles the aminoacyl-adenyl terminus of aminoacyl-tRNA and competes for binding to the large ribosomal subunit, which causes termination. A dilution of 1:1000 is recommended for inducing apoptosis in most cell types, but the optimal dose may vary for different cell types and culture conditions; please consult published literature to determine optimal dilution and incubation time for individual samples. Set contains 100 µl each of the following components:

Components
• Aphidicolin (Cat. No. KS13301-100UL), (10 µg/µl in DMSO)
• Betulinic Acid (Cat. No. KS13302-100UL), (20 µg/µl in DMSO)
• Paclitaxel, Taxus brevifolia (Cat. No. KS13303-100UL), (2 µg/µl in DMSO)
• Staurosporine, Streptomyces sp. (Cat. No. KS13304-100UL), (0.5 µg/µl in DMSO)
• Doxorubicin, Hydrochloride (Cat. No. KS13305-100UL), (5 µg/µl in H2O)
• Puromycin, Dihydrochloride (Cat. No. KS13306-100UL), (20 µg/µl in H2O)
FormLiquid
Formulation100 µl each inhibitor in DMSO or H₂O
Intert gas (Yes/No) Packaged under inert gas
Storage Protect from light
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot, purge with inert gas, and freeze (-20°C). Once opened, stock solutions are stable for up to 3 months when stored under the indicated conditions.
Toxicity Multiple Toxicity Values, refer to MSDS
ReferencesLing, Y., et al. 2001. ol Pharmacol. 59, 593.
Yang, X.H, et al. 2001. Cancer Res. 61, 348.
Sanwal, V., et al. 2001. Exp. Mol. Pathol. 70, 54.
Fulda, S., and Debatin, K.M. 2000. Med. Pediatr. Oncol. 35, 616.
Shim, Y.H., et al. 2000. Int. J. Hematol. 72, 484.
Sangfelt, O., et al. 1997. Cell Growth Differ. 8, 343.