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189483 Autocamtide-3

189483
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





A synthetic substrate specific for Ca2+/calmodulin-dependent protein kinase II (CaMK II). The substrate sequence is based on the CaMKII pseudosubstrate core found within the autoinhibitory domain of the enzyme. Autophosphorylation of Thr286 within the autoinhibitory domain traps bound calmodulin by greatly reducing its dissociation rate, thus prolonging the active state.
Catalogue Number189483
Brand Family Calbiochem®
SynonymsAC-3, KKALHRQETVDAL
References
ReferencesFluck, M., et al. 2000. J. Appl. Physiol. 88, 352.
Yang, E., and Schulman, H. 1999. J. Biol. Chem. 274, 26199.
Kahn, E.S., et al. 1998. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 119, 739.
Product Information
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Hill FormulaC₆₅H₁₁₃N₂₁O₂₀
Chemical formulaC₆₅H₁₁₃N₂₁O₂₀
Hygroscopic Hygroscopic
Applications
Biological Information
Purity≥98% by HPLC
Physicochemical Information
Peptide SequenceH-Lys-Lys-Ala-Leu-His-Arg-Gln-Glu-Thr-Val-Asp-Ala-Leu-OH
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Autocamtide-3 MSDS

Title

Safety Data Sheet (SDS) 

Autocamtide-3 Certificates of Analysis

TitleLot Number
189483

References

Přehled odkazů
Fluck, M., et al. 2000. J. Appl. Physiol. 88, 352.
Yang, E., and Schulman, H. 1999. J. Biol. Chem. 274, 26199.
Kahn, E.S., et al. 1998. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 119, 739.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-April-2008 RFH
SynonymsAC-3, KKALHRQETVDAL
DescriptionA synthetic substrate specific for Ca2+/calmodulin-dependent protein kinase II (CaMKII). The substrate sequence is based on the CaMKII pseudosubstrate core found within the auto-inhibitory domain of the enzyme. Autophosphorylation of Thr286 within the auto-inhibitory domain traps bound calmodulin by greatly reducing its dissociation rate, thus prolonging the active state.
FormLyophilized
FormulationSupplied as a trifluoroacetate salt.
Recommended reaction conditionsMaximal CaMKII activity has been measured in reaction mixtures containing 50 mM PIPES, pH 7.0, 10 mM MgCl2, 0.1 mg/ml BSA, 0.5 mM CaCl2, 50 µM ATP containing [γ-32P]ATP, 1 µM calmodulin, and 20 µM Autocamtide-3.
Chemical formulaC₆₅H₁₁₃N₂₁O₂₀
Peptide SequenceH-Lys-Lys-Ala-Leu-His-Arg-Gln-Glu-Thr-Val-Asp-Ala-Leu-OH
Purity≥98% by HPLC
SolubilityH₂O (1 mg/ml)
Storage -20°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesFluck, M., et al. 2000. J. Appl. Physiol. 88, 352.
Yang, E., and Schulman, H. 1999. J. Biol. Chem. 274, 26199.
Kahn, E.S., et al. 1998. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 119, 739.