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203644 BMP-6, Mouse, Recombinant, NSO Cells

203644
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Purity
≥95% by SDS-PAGE
Description
Overview

This product has been discontinued.





Recombinant, mouse bone morphogenetic protein-6 expressed in NSO cells. An osteogenic protein belonging to the transforming growth factor-β (TGF-β) superfamily. BMP-6 has been shown to strongly induce phosphorylation and nuclear accumulation of Smad5. BMPs have also been shown to be involved in embryogenesis and morphogenesis of cells of osteoblastic lineage. A positive regulator of keratinocyte differentiation. MW: 18000 + 23000 (reducing conditions).
M.W. 18,000 and 23,000 under reducing conditions.
Catalogue Number203644
Brand Family Calbiochem®
SynonymsBone Morphogenetic Protein-6
References
ReferencesMcDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
Raida, M., et al. 1999. Int. J. Cancer 83, 38.
Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
Reddi, A.H. 1998. Nature Biotech. 16, 247.
Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.
Product Information
FormLyophilized
FormulationLyophilized from a 0.2 µm filtered solution of 35% ACN, 0.1% TFA, 50 µg BSA/µg BMP-6.
Applications
Biological Information
Biological activityED₅₀ = 50-150 ng/ml based on the ability to induce alkaline phosphatase expression in MC3T3-E1 cells
Purity≥95% by SDS-PAGE
Physicochemical Information
Contaminantsendotoxin: ≤100 pg/µg cytokine
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 23/24/25-36/37/38

Toxic by inhalation, in contact with skin and if swallowed.
Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36/37/39-45

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

BMP-6, Mouse, Recombinant, NSO Cells Certificates of Analysis

TitleLot Number
203644

References

Přehled odkazů
McDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
Raida, M., et al. 1999. Int. J. Cancer 83, 38.
Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
Reddi, A.H. 1998. Nature Biotech. 16, 247.
Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-May-2008 RFH
SynonymsBone Morphogenetic Protein-6
DescriptionRecombinant, mouse bone morphogenetic protein-6 expressed in NSO cells. An osteogenic protein belonging to the transforming growth factor β (TGF-β) superfamily. A positive regulator of keratinocyte differentiation. BMP-6 has been shown to strongly induce phosphorylation and nuclear accumulation of Smad5. Can be used for tumor cell differentiation and progression. MW: 18000 + 23000 (reducing conditions).
FormLyophilized
FormulationLyophilized from a 0.2 µm filtered solution of 35% ACN, 0.1% TFA, 50 µg BSA/µg BMP-6.
Purity≥95% by SDS-PAGE
Contaminantsendotoxin: ≤100 pg/µg cytokine
Biological activityED₅₀ = 50-150 ng/ml based on the ability to induce alkaline phosphatase expression in MC3T3-E1 cells
SolubilityReconstitute at a concentration of at ≥50 µg/ml in sterile PBS containing at least 0.1% HSA or BSA.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity Standard Handling
ReferencesMcDonnell, M.A., et al. 2001. Exp. Cell. Res. 263, 265.
Ebisawa, T., et al. 1999. J. Cell Sci. 112, 3519.
Raida, M., et al. 1999. Int. J. Cancer 83, 38.
Ebendal, T., et al. 1998. J. Neurosci. Res. 51, 139.
Reddi, A.H. 1998. Nature Biotech. 16, 247.
Honda, Y., et al. 1997. Calcif. Tissue Int. 60, 297.