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200497 Betacellulin, Mouse, Recombinant

200497
  
Purchase on Sigma-Aldrich

Přehled

Replacement Information

Tabulka spec. kláve

Purity
≥97% by SDS-PAGE
Description
Overview

This product has been discontinued.



Recombinant, mouse betacellulin (BTC) cinsisting of amino acids 32-118 and expressed in E. coli. Mouse BTC is 93% identical to the amino acid sequence of rat and 79% with that of human. BTC, a heparin-binding protein, is expressed in tissues such as uterus, liver, pancreas, and kidney and promotes pancreatic beta-cell growth and differentiation. Also known as a potent mitogen for retinal pigment epithelial cells, fibroblasts, and vascular smooth muscle cells.

Catalogue Number200497
Brand Family Calbiochem®
References
ReferencesBastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
Li, L., et al. 2001. Endocrinology 142, 5379.
Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
Pathak, B.G., et al. 1995. Genomics 28, 116.
Shing, Y., et al. 1993. Science 259, 1604.
Product Information
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityED₅₀ = 0.15-0.6 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
Purity≥97% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 Eu/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Betacellulin, Mouse, Recombinant MSDS

Title

Safety Data Sheet (SDS) 

Betacellulin, Mouse, Recombinant Certificates of Analysis

TitleLot Number
200497

References

Přehled odkazů
Bastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
Li, L., et al. 2001. Endocrinology 142, 5379.
Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
Pathak, B.G., et al. 1995. Genomics 28, 116.
Shing, Y., et al. 1993. Science 259, 1604.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision19-Sepmtember-2011 RFH
DescriptionRecombinant, mouse betacellulin (BTC) cinsisting of amino acids 32-118 and expressed in E. coli. Mouse BTC is 93% identical to the amino acid sequence of rat and 79% with that of human. BTC, a heparin-binding protein, is expressed in tissues such as uterus, liver, pancreas, and kidney and promotes pancreatic beta-cell growth and differentiation. Also known as a potent mitogen for retinal pigment epithelial cells, fibroblasts, and vascular smooth muscle cells.
FormLiquid
FormulationLyophilized from 0.2 µm sterile-filtered PBS, BSA.
Purity≥97% by SDS-PAGE
ContaminantsEndotoxin: <1.0 Eu/µg protein
Biological activityED₅₀ = 0.15-0.6 ng/ml as measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblasts.
SolubilityReconstitute to 10 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesBastian, S.E., et al. 2001. J. Endocrinol. 168, 203.
Li, L., et al. 2001. Endocrinology 142, 5379.
Dunbar, A.J. and C. Goddard. 2000. Int. J. Biochem. Cell Biol. 32, B805.
Tada, H., et al. 1999. J. Cell Biochem. 72, 423.
Pathak, B.G., et al. 1995. Genomics 28, 116.
Shing, Y., et al. 1993. Science 259, 1604.