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SCT064 BioTracker™ NTP-Transporter Molecule

SCT064
1 mg  
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Replacement Information
Description
Catalogue NumberSCT064
DescriptionBioTracker™ NTP-Transporter Molecule
OverviewNucleoside triphosphates (NTPs) are important metabolites that are involved in numerous cellular processes. However, due to their anionic nature, NTPs do not readily permeate cell membranes. To overcome this barrier, synthetic analogues that are neutral and more readily transportable are commonly used in cell-based assays. However, these synthetic analogues have a very low efficiency of conversion to active metabolites. Hence there is a need to optimize transport of NTPs into the cells.

BioTracker™ NTP-Transporter Molecule has a receptor site, for selectively binding to the NTPs, and a cell penetrating agent. The transporter can bind to NTPs and quickly move them into cells without any apparent damage to the cell membrane or decrease in cell viability. The transported NTP is rapidly liberated from the complex and available in the nuclei for incorporation into DNA. Labeled NTPs can be used for live cell imaging, cell proliferation assays, synthetic biology studies, synthesizing artificial DNA, tracking cell division, research on active antiviral and anticancer nucleotides, among other things.

References
Zawad Z et al. Transport of Nucleoside Triphosphates into Cells by Artificial Molecular Transporters. Angew. Chem. Int. Ed. 2018. 57. 9891-9895.
Alternate Names
  • Live cell imaging probe
  • Cell cylce probe
References
Product Information
Detection methodFluorescence
PresentationLyophilized
Applications
ApplicationQuick and efficient transport of labeled NTPs into cells for live cell imaging of DNA replication, synthesis, etc. Labeling of DNA with fluorescent NTPs is one of the essential methods in cell cycle analysis.
Key Applications
  • Cell Based Assays
Application NotesLive cell fluorescent imaging
Biological Information
ConcentrationPlease refer to lot specific datasheet.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssurancePurity: ≥ 98% confirmed by HNMR, LC-MS and HPLC and elemental analysis
Molar Mass: 3470.14 g/mol
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore BioTracker™ NTP-Transporter Molecule at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications

Documentation

BioTracker™ NTP-Transporter Molecule MSDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Flow cytometric determination of cell cycle progression via direct labeling of replicated DNA
Erika Kužmová 1 , Zbigniew Zawada 2 , Michal Navrátil 1 , Jana Günterová 1 , Tomáš Kraus 3
Anal Biochem  614  614:114002  2020

Zobrazit abstrakt
33159846 33159846

Data Sheet

Title
BioTracker™ NTP-Transporter Molecule