324691 Elastatinal - CAS 51798-45-9 - Calbiochem

324691
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      Přehled

      Replacement Information

      Tabulka spec. kláve

      Empirical FormulaCAS #
      C₂₁H₃₆N₈O₇ 51798-45-9

      Ceny a dostupnost

      Katalogové číslo DostupnostBalení ks/bal. Cena Množství
      324691-5MG
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          Plastová ampulka 5 mg
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          Vyžádat nacenění
          Description
          OverviewAn inhibitor of elastase-like serine protease produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Inhibitory activity: ~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
          Catalogue Number324691
          Brand Family Calbiochem®
          References
          ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
          Umezawa, H. 1976. Methods Enzymol. 55, 678.
          Product Information
          CAS number51798-45-9
          ATP CompetitiveN
          FormYellow solid
          Hill FormulaC₂₁H₃₆N₈O₇
          Chemical formulaC₂₁H₃₆N₈O₇
          ReversibleN
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetElastase-like serine protease produced by actinomycetes
          Primary Target IC<sub>50</sub>~0.5 µg/ml using Suc-(Ala)3-pNA as a substrate at 25°C, pH 8.0.
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSLZ9600000
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Elastatinal - CAS 51798-45-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          324691

          References

          Přehled odkazů
          Menendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
          Umezawa, H. 1976. Methods Enzymol. 55, 678.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-June-2008 RFH
          DescriptionElastase-like serine protease inhibitor produced by actinomycetes. Inhibits PMSF-stimulated degradation of histone H1 and HMG proteins. Also inhibits α2-macroglobulin-protease complexes. Effective at concentrations of 10-100 µM.
          FormYellow solid
          Recommended reaction conditions
          Assay Procedure 1. Dissolve enzyme in reaction buffer: 100 mM Tris-HCl, pH 7.5, 500 mM NaCl. 2. Prepare the substrate stock solution by dissolving Elastase Substrate I (MeOSuc-Ala-Ala-Pro-Val- pNA, Cat. No. 324696) at 50 mM in DMSO. 3. Just prior to assay, dilute the substrate stock solution with an equal volume of 200 mM Tris-HCl, pH 8.0. Mix well. 4. Prepare the assay mixture by combining 960 µl of reaction buffer with 40 µl of diluted substrate stock solution. 5. Add enzyme to assay mixture, mix, and incubate at 25°C. 6. Monitor the change in absorbance at 410 nm. Use an e of 8800 at 410 nm to calculate the hydrolysis of nitroanilide.
          CAS number51798-45-9
          RTECSLZ9600000
          Chemical formulaC₂₁H₃₆N₈O₇
          SolubilityH₂O (5 mg/ml)
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesMenendez-Arias, L. 1992. J. Biol. Chem. 267, 11392.
          Umezawa, H. 1976. Methods Enzymol. 55, 678.