325902 FKBP12, GST-Fusion, Human, Recombinant, E. coli

325902
  
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      Přehled

      Replacement Information
      Description
      OverviewFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.

      One vial is enough for inhibitor screening of four K-LISA™ mTOR Activity Kits (CBA055) (400 wells).Upon initial thawing, aliquot and store at -70° C.
      Catalogue Number325902
      Brand Family Calbiochem®
      SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
      Application Data
      The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
      References
      ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.
      Product Information
      FormLiquid
      FormulationIn PBS, 10% glycerol, pH 7.2.
      Quality LevelMQ100
      Applications
      ApplicationFKBP12, GST-Fusion, Human, Recombinant, E. coli is a full-length protein that is fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506.
      Biological Information
      Purity≥90% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FKBP12, GST-Fusion, Human, Recombinant, E. coli MSDS

      Title

      Safety Data Sheet (SDS) 

      FKBP12, GST-Fusion, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      325902

      References

      Přehled odkazů
      Jin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2007 JSW
      SynonymsFK506 binding protein 1A, 12 kDa, FKBP1A
      Application Data
      The activity of mTOR was assessed without inhibitor and in the presence of rapamycin alone (Cat. No. 553210), FKBP12 alone (Cat. No. 325902), FKBP12/rapamycin complex, and Wortmannin (Cat. No. 681675), using the K-LISA™ mTOR Activity Kit (Cat. No. CBA055) as outlined in the Detailed Protocol. The FKBP12/rapamaycin inhibitor complex was pre-assembled by mixing 62.5 µg/ml FKBP12 and 50 µM rapamycin on ice for 10 min. The mTOR Standard (50 µl) was incubated for 15 min on ice with FKBP12 (2 µl: final concentration 2.5 µg/ml), rapamycin (2 µl; final concentration 2 µM). FKPP12/rapamycin complex (final concentration 2.5 µg/ml, 2 µM rapamycin) or wortmannin (final concentration 10 µM). The kinase reaction was initiated by transferring each mixture to a corresponding well containing 2X kinase buffer and ATP. The results were assessed as outlined in the user protocol.
      DescriptionFull-length recombinant, human FK506 binding protein 12-rapamycin associated protein 1 (FKBP12) (accession number = NP000792) fused to GST at the N-terminus and expressed in E.coli. FKBP12 is a cytosolic receptor for both rapamycin and FK506 that also serves as a natural cofactor for rapamycin's inhibition of mTOR. The molecular weight of 38,000 Da includes the GST moiety.
      FormLiquid
      FormulationIn PBS, 10% glycerol, pH 7.2.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥90% by SDS-PAGE
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesJin, Y.J., et al. 1991. Proc Natl Acad Sci USA 88, 6677.