371739 G-Proteins, Bovine Brain, Functional

371739
  
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      Přehled

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies of G-proteins. Contains the following heterotrimeric G-proteins: Goα (4 - 5 μM), Giα-1 (1 - 2 μM), Giα-2 (1 - 2 μM), and Giα-3 (<1 μM). The βγ-subunit complex associated with these α-subunits represents the predominant form found in bovine brain. Characterized by GTPγS binding activity and by immunoblotting using subunit-specific antisera.

      Catalogue Number371739
      Brand Family Calbiochem®
      References
      ReferencesCasey, P., et al. 1991. Methods Enzymol. 195, 315.
      Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
      Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.
      Product Information
      FormLiquid
      FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.1% LUBROL® Detergent, pH 7.6.
      Applications
      ApplicationG-Proteins, Bovine Brain, Functional, is a mixture of purified pertussis toxin-sensitive G-proteins from bovine brain. Suitable for functional studies. Contains Goα, Giα-1, Giα-2, and Giα-3.
      Key Applications Immunoblotting (Western Blotting)
      Application NotesSuitable for GTPγS binding assay and Immunoblotting
      Application CommentsCharacterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      G-Proteins, Bovine Brain, Functional Certificates of Analysis

      TitleLot Number
      371739

      References

      Přehled odkazů
      Casey, P., et al. 1991. Methods Enzymol. 195, 315.
      Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
      Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.

      Citations

      Název
    • Rodrigo Cunha, M Dolores Constantino and J Alexandre Ribeiro. (1999) G Protein coupling of CGS 21680 binding sites in the rat hippocampus and cortex is different from that of adenosine A1 and striatal A2A receptors.. Naunyn-Schmiedeberg's Archives of Pharmacology 359, 295-302.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision02-August-2007 RFH
      ApplicationSuitable for GTPγS binding assay and Immunoblotting
      DescriptionMixture of purified pertussis toxin-sensitive G proteins from bovine brain useful for functional studies. Contains the following heterotrimeric G proteins: Goα (approximately 4-5 µM), Giα-1 (approximately 1-2 µM), Giα-2 (approximately 1-2 µM), and Giα-3 (<1 µM). The βγ subunit complex (approximately 8 µM) associated with these α subunits represents the predominant form found in bovine brain.
      FormLiquid
      FormulationIn 50 mM HEPES, 1 mM DTT, 1 mM EDTA, 0.1% LUBROL® Detergent, pH 7.6.
      Concentration Label Please refer to vial label for lot-specific concentration
      CommentsCharacterized by GTPγS binding and in immunoblot using subunit-specific antisera. Titration is recommended.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C)
      Toxicity Standard Handling
      ReferencesCasey, P., et al. 1991. Methods Enzymol. 195, 315.
      Linder and Gilman, A.G. 1991. Methods Enzymol. 195, 207.
      Goldsmith, P.P., et al. 1987. J. Biol. Chem. 262, 14,683.
      Citation
    • Rodrigo Cunha, M Dolores Constantino and J Alexandre Ribeiro. (1999) G Protein coupling of CGS 21680 binding sites in the rat hippocampus and cortex is different from that of adenosine A1 and striatal A2A receptors.. Naunyn-Schmiedeberg's Archives of Pharmacology 359, 295-302.