345845 GGTase Substrate, Fluorogenic

345845
  
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      Přehled

      Replacement Information
      Description
      OverviewA pentapeptide based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Serves as a fluorescent substrate for continuously monitoring fluorescent protein-geranylgeranyltransferase (type I) (GGTase-I) activity (Km = 5 µM) in the presence of geranylgeranyl diphosphate. S-geranylgeranylation of the cysteine moiety results in a significant enhancement in fluorescence intensity (monitored at λ = 460 nm). Reported to be highly selective for GGTase and is not recognized by FTase.
      Catalogue Number345845
      Brand Family Calbiochem®
      SynonymsDansyl-GCVLL, Dns-GCVLL
      References
      ReferencesClausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
      Product Information
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₃₄H₅₂N₆O₈S₂
      Chemical formulaC₃₄H₅₂N₆O₈S₂
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      GGTase Substrate, Fluorogenic Certificates of Analysis

      TitleLot Number
      345845

      References

      Přehled odkazů
      Clausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-December-2008 RFH
      SynonymsDansyl-GCVLL, Dns-GCVLL
      DescriptionA pentapeptide substrate based on the carboxyl terminus of H-Ras with a dansyl group attached to the amino terminus. Useful for continuous fluorescent monitoring of protein geranylgeranyltransferase (type I) (GGTase-I) activity in the presence of geranylgeranyl diphosphate (Km = 5 µM). S-geranylgeranylation of the cysteine residue results in a significant enhancement of fluorescence intensity at a wavelength of 460 nm. Reported to be highly specific for GGTase-I and is not recognized by protein farnesyltransferase (FTase). Excitation maximum: ~360 nm; emission maximum: ~460 nm.
      FormLyophilized
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Recommended reaction conditions10 µM geranylgeranyl diphosphate, 10 µM Dansyl-GCVLL, 50-200 ng GGTase-I in 50 mM Tris-HCl, pH 7.5, 50 mM ZnCl2, 5 mM MgCl2, 1 mM DTT, 20 mM KCl, and 0.2% octyl-β-D-glucopyranoside.
      Chemical formulaC₃₄H₅₂N₆O₈S₂
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (10 mg/ml)
      Storage -20°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesClausen, V.A., et al. 2001. Biochemistry 40, 3920. Zhang, F.L., and Casey, P.J. 1996. Biochem. J. 320, 925. Pickett, W.C., et al. 1995. Anal. Biochem. 225, 60.