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119109 Luciferin-Luciferase

119109
  
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Přehled

Replacement Information
Description
Overview

This product has been discontinued.





Firefly luciferin-luciferase reagent for the ATP assay. Essential component of the ATP Assay Kit (Cat. No. 119107). Luciferase catalyzes the oxidation of D-luciferin to oxyluciferin in the presence of ATP and oxygen. As the reaction proceeds, light is produced.
Catalogue Number119109
Brand Family Calbiochem®
References
ReferencesDeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.
Product Information
Activity≥3,200,000 RLU's per 10 µl reconstituted solution at 2 x 10⁻⁸ M ATP using a Monolight 3010 Luminometer
FormLyophilized
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage ≤ -70°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C or for up to 8 h at 4°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Luciferin-Luciferase Certificates of Analysis

TitleLot Number
119109

References

Přehled odkazů
DeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision08-April-2008 RFH
DescriptionFirefly luciferin-luciferase reagent for the ATP assay. Essential component of the ATP Assay Kit (Cat. No. 119107). Luciferase catalyzes the oxidation of D-luciferin to oxyluciferin in the presence of ATP and oxygen. As the reaction proceeds, light is produced.
FormLyophilized
Activity≥3,200,000 RLU's per 10 µl reconstituted solution at 2 x 10⁻⁸ M ATP using a Monolight 3010 Luminometer
SolubilityReconstitute in 500 µl 25 mM HEPES buffer, 10 mM MgCl₂, 0.02% NaN₃, pH 7.5.
Storage ≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 month at -70°C or for up to 8 h at 4°C.
Toxicity Standard Handling
ReferencesDeLuca, M., et al. 1979. Anal. Biochem. 95, 194.
Chappelle, E., et al. 1978. Methods Enzymol. 57, 65.