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ABS45 Anti-phospho-MYPT1 (Thr696) Antibody

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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, PIARbAffinity PurifiedPolyclonal Antibody
      Catalogue NumberABS45
      DescriptionAnti-phospho-MYPT1 (Thr696) Antibody
      Alternate Names
      • Myosin phosphatase target subunit 1
      • Myosin phosphatase-targeting subunit 1
      • Protein phosphatase myosin-binding subunit
      • myosin phosphatase, target subunit 1
      • protein phosphatase 1, regulatory (inhibitor) subunit 12
      Background InformationProtein phosphatase 1 regulatory subunit 12A (UniProt: O14974; also known as Myosin phosphatase-targeting subunit 1, Myosin phosphatase target subunit 1, Protein phosphatase myosin-binding subunit) is encoded by the PPP1R12A (also known as MBS, MYPT1) gene (Gene ID: 4659) in human. Myosin light chain phosphatase with its regulatory subunit, myosin phosphatase target subunit 1 (MYPT1), modulates Ca2+-dependent phosphorylation of myosin light chain by myosin light chain kinase, which is essential for smooth muscle contraction. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho, which inhibits myosin phosphatase through the action of Rho-kinase. MYPT1 is localized on stress fibers and is distributed close to the cell membrane and at cell-cell contacts to regulate myosin phosphatase activity. It is phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696 that inhibits PP1C activity by an intramolecular mechanism. Mutations in PPP1R12A are involved in Genitourinary and/or brain malformation syndrome (GUBS), an autosomal dominant syndrome that is characterized by multiple congenital anomalies including urogenital malformations and brain abnormalities. Five isoforms of MYPT1 have been described that are produced by alternative splicing. (Ref.: Qiao, Y-N., et al. (2014). J. Biol. Chem. 289(32); 22512-22523).
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      • Calyculin/Okadaic treated and untreated HEK293 cell lysates
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
      Quality LevelMQ100
      ApplicationAnti-phospho-MYPT1 (Thr696), Cat. No. ABS45, is a rabbit polyclonal antibody that detects MYPT1 phosphorylated on Threonine 696 and is tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Western Blotting
      • Peptide Inhibition Assay
      Application NotesPeptide Inhibition Assay: Target band detection in a lysate from HeLa cells treated with Calyculin (50 nM) and Okadaic Acid (500 nM) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 11 amino acids surrounding phosphothreonine 696 of human MYPT1.
      EpitopePhosphorylated Thr696
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      SpecificityThis rabbit polyclonal antibody specifically detects MYPT1 phosphorylated on threonine 696.
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to react with Xenopus, Rat, Canine, Primate, Chicken, Mouse, Horse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMyosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small
      guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells.The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of
      phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA,
      phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
      Gene Symbol
      • MBS
      • MGC133042
      • MYPT1
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Regulates myosin phosphatase activity.
      SUBUNIT STRUCTURE: PP1 comprises a catalytic subunit, PPP1CA, PPP1CB or PPP1CC, and one or several targeting or regulatory subunits. PPP1R12A mediates binding to myosin. Interacts with ARHA and CIT By similarity. Binds PPP1R12B, ROCK1 and IL16.
      SUBCELLULAR LOCATION: Cytoplasm. Note: Along actomyosin filaments and stress fibers.
      PTM: Phosphorylated by CIT (Rho-associated kinase) By similarity. Phosphorylated cooperatively by ROCK1 and CDC42BP on Thr-696. Phosphorylated on upon DNA damage, probably by ATM or ATR.
      SEQUENCE SIMILARITIES: Contains 6 ANK repeats.
      Molecular Weight~130 kDa observed; 115.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cells treated with Calyculin and Okadaic Acid.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected phosphorylated MYPT1 (Thr696) in lysate from HeLa cells treated for 30 minutes with Calyculin (50 nM) and Okadaic Acid (500 nM).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size200 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Bestellnummer GTIN
      ABS45 04053252280634


      Anti-phospho-MYPT1 (Thr696) Antibody SDB


      Sicherheitsdatenblatt (SDB) 

      Anti-phospho-MYPT1 (Thr696) Antibody Analysenzertifikate

      Anti-phospho-MYPT1 (Thr696) - 2121780 2121780
      Anti-phospho-MYPT1 (Thr696) - 2377421 2377421
      Anti-phospho-MYPT1 (Thr696) - 2452524 2452524
      Anti-phospho-MYPT1 (Thr696) - 2181279 2181279
      Anti-phospho-MYPT1 (Thr696) - 2290259 2290259
      Anti-phospho-MYPT1 (Thr696) - 2476996 2476996
      Anti-phospho-MYPT1 (Thr696) - 3194632 3194632
      Anti-phospho-MYPT1 (Thr696) - 3383154 3383154
      Anti-phospho-MYPT1 (Thr696) - 3610571 3610571
      Anti-phospho-MYPT1 (Thr696) - 3733837 3733837


      ÜbersichtPub Med ID
      Defining the phospho-adhesome through the phosphoproteomic analysis of integrin signalling.
      Robertson, J; Jacquemet, G; Byron, A; Jones, MC; Warwood, S; Selley, JN; Knight, D; Humphries, JD; Humphries, MJ
      Nature communications  6  6265  2015

      Abstract anzeigen
      25677187 25677187
      Inactivation of the Hippo tumour suppressor pathway by integrin-linked kinase.
      Serrano, I; McDonald, PC; Lock, F; Muller, WJ; Dedhar, S
      Nature communications  4  2976  2013

      Abstract anzeigen
      24356468 24356468
      Distinct roles for ROCK1 and ROCK2 in the regulation of cell detachment.
      Shi, J; Wu, X; Surma, M; Vemula, S; Zhang, L; Yang, Y; Kapur, R; Wei, L
      Cell death & disease  4  e483  2013

      Abstract anzeigen
      23392171 23392171
      MRP4-mediated regulation of intracellular cAMP and cGMP levels in trabecular meshwork cells and homeostasis of intraocular pressure.
      Pattabiraman, PP; Pecen, PE; Rao, PV
      Investigative ophthalmology & visual science  54  1636-49  2013

      Abstract anzeigen
      23385799 23385799
      Mechanisms underlying potentiation of endothelin-1-induced myofilament Ca(2+) sensitization after subarachnoid hemorrhage.
      Kikkawa, Y; Matsuo, S; Kameda, K; Hirano, M; Nakamizo, A; Sasaki, T; Hirano, K
      Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism  32  341-52  2011

      Abstract anzeigen
      21952110 21952110
      The role of calcium-independent phospholipase A2γ in modulation of aqueous humor drainage and Ca2+ sensitization of trabecular meshwork contraction.
      Pattabiraman, PP; Lih, FB; Tomer, KB; Rao, PV
      American journal of physiology. Cell physiology  302  C979-91  2011

      Abstract anzeigen
      22237407 22237407
      RKI-1447 is a potent inhibitor of the Rho-associated ROCK kinases with anti-invasive and antitumor activities in breast cancer.
      Patel, RA; Forinash, KD; Pireddu, R; Sun, Y; Sun, N; Martin, MP; Schönbrunn, E; Lawrence, NJ; Sebti, SM
      Cancer research  72  5025-34  2011

      Abstract anzeigen
      22846914 22846914
      Rho-kinase inhibition alleviates pulmonary hypertension in transgenic mice expressing a dominant-negative type II bone morphogenetic protein receptor gene.
      Yasuda, T; Tada, Y; Tanabe, N; Tatsumi, K; West, J
      American journal of physiology. Lung cellular and molecular physiology  301  L667-74  2010

      Abstract anzeigen
      21856816 21856816


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      Life Science Research > Antibodies and Assays > Primary Antibodies