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71491 Overnight Express™ Instant TB Medium - Novagen

71491
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Overview

Replacement Information

Products

Catalogue NumberPackaging Qty/Pack
71491-3 Plastic ampoule 1 easy
71491-4 Glass bottle 5 easy
71491-5 Plastic ampoule 1 kg
Description
Overview

Overnight Express™ Instant TB Medium is a granulated autoinduction culture medium for high-level protein production in pET and other IPTG-inducible bacterial expression systems. Instant TB Medium is a combination of Overnight Express Autoinduction System 1 components (Cat. No. 71300) with rich Terrific Broth (TB) medium and is supplied in two formats.
EasyPak is an aluminum foil pouch containing 60 g granulated medium, sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min to dissolve. Overnight Express Instant TB Medium is also available in 1 kg bottles. Granulated medium ensures rapid, uniform dissolution in water, prevents clumping of the medium, and avoids inhalation of airborne powder.

Overnight Express™ Autoinduction media are based on technology developed by F. William Studier at Brookhaven National Laboratory and enable regulated protein expression in E. coli,without monitoring the culture or adding IPTG during cell growth. The method uses media components that are metabolized differentially to promote growth to high cell density and automatically induce protein expression from lac promoters. As a result, these unique autoinduction media provide high-level protein production in pET and other IPTG-inducible bacterial expression systems.

Cell mass and target protein yield are often increased several-fold compared to conventional protocols using induction with IPTG. Autoinduction media are extremely convenient for routine expression of proteins in multiple cultures and are ideal for high-throughput parallel analysis of protein expression, solubility, and purification from multiple expression clones




EasyPak is an aluminum foil pouch containing 60 g granulated medium sufficient for 1 L culture. Just add the EasyPak contents to 1 L sterile water, supplement with 10 ml glycerol, and microwave for 2 min. Overnight Express Instant TB Medium is also available in 1 kg bottles.


In addition to Instant TB Medium, a variety of Overnight Express Autoinduction Media are available for different methods:

Overnight Express™ Autoinduction System 1 (71300) contains 3 sterile solutions, which can be added to your choice of glucose-free bacterial growth medium.

Overnight Express™ Autoinduction System 2 (71366) prepares a defined bacterial growth medium that can be used to selenomethionine-label proteins for x-ray crystallography.


Overnight Express Autoinduction NMR Media are 3 different media designed for cell growth Optimization (71760), 15N (71759) protein labeling, and 15N, 13C (71789) dual protein labeling for NMR analysis.
PRODUCT USE RESTRICTIONS - PRODUCT LABEL LICENSE

The Overnight Express™ Auto Induction Media material (Products) includes patented technology embodied therein developed at Brookhaven National Laboratory under contract with the U.S. Department of Energy. The patent applications or issued patents embodied in this Product were assigned to Brookhaven Science Associates, LLC (BSA). BSA has enabled EMD Millipore and its Affiliates to grant a non-exclusive license for use of this patented technology, including use with the enclosed Products, based upon your acceptance of the following terms:
1. These Products are to be used for non-commercial research purposes only. For the avoidance of doubt, such non-commercial research purposes include research use by academic, non-profit, and commercial entities.
2. A separate license is required for any commercial use, including the use of the enclosed Products for developing or making other materials for sale to third parties.
3. Information about licenses for commercial use of the Products may be obtained from:

Office of Technology Commercialization and Partnerships
Brookhaven National Laboratory
Building No. 490C - P.O. Box 5000
Upton, New York 11973-5000
Telephone: 631-344-4711
Fax: 631-344-3729
E-mail: pupadhya@bnl.gov


4. Information about licenses for commercial manufacture and/or sale of the Products may be obtained from:

Intellectual Property Department
EMD Millipore Corporation
28820 Single Oak Drive
Temecula, CA 92590
E-mail: licensing@milliporesigma.com


5. No Products that contain this patented technology may be transferred or distributed further to third parties outside of your laboratory, unless the recipient third party receives a license from BSA and agrees to be bound by its terms.

By keeping or using the enclosed Product(s), you accept the above terms of use solely for noncommercial research purpose. If you refuse to accept the terms herein, you must return the Product(s) to EMD Millipore.
Catalogue Number71491
Brand Family Novagen®
References
Product Information
Quality LevelMQ200
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +15°C to +30°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Overnight Express™ Instant TB Medium - Novagen Certificates of Analysis

TitleLot Number
71491

Brochure

Title
Overnight Express Autoinduction Systems

Citations

Title
  • Erika J. Mancini, et al. (2007) Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 A resolution. Protein Science 16, 2294-2300.
  • Liguo Zhang, Kenneth P. Nephew and Patricia J. Gallagher. (2007) Regulation of death-associated protein kinase: stabilization by HSP90 heterocomplexes. Journal of Biological Chemistry 282, 11795-11804.
  • User Protocols

    Title
    TB526 Overnight Express™ Instant LB and TB Media