C0549
Chloroform:Isoamyl alcohol 24:1
suitable for nucleic acid purification
Synonym(s):
chloroform-isoamyl alcohol
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About This Item
NACRES:
NA.52
UNSPSC Code:
12190000
form
liquid
Quality Level
contains
0.6-1.0% ethanol as stabilizer
suitability
suitable for nucleic acid purification
application(s)
agriculture
General description
Chloroform:Isoamyl Alcohol 24:1 is an organic reagent used for DNA extraction and precipitation.
Application
Chloroform:Isoamyl alcohol 24:1 has been used:
- to extract bacterial genomic DNA, DNA from Daphnia spp, and salmon sperms.
- for a single DNA precipitation step for high-quality DNA extraction.
- for protein extraction and RNAse treatment.
- in phenolic oxidation to separate phenolics from the aqueous portion.
- for RNA isolation from brown adipose tissue (BAT), inguinal white adipose tissue (iWAT), and epididymal adipose tissue (eWAT).
- in modified cetyltrimethyl ammonium bromide (CTAB) method to extract total RNA from Impatiens balsamina.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Carc. 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 Oral - STOT SE 3
target_organs
Central nervous system, Liver,Kidney
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk
WGK 3
flash_point_f
185.0 °F
flash_point_c
85 °C
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