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A3678

Ammonium persulfate

Molecular Biology, suitable for electrophoresis, ≥98%

Synonym(s):

AP, APS, Ammonium peroxodisulfate, Ammonium peroxydisulfate, PER

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About This Item

Linear Formula:
(NH4)2S2O8
CAS Number:
7727-54-0
Molecular Weight:
228.20
PubChem Substance ID:
24890800
eCl@ss:
38030421
UNSPSC Code:
41105319
NACRES:
NB.22
EC Number:
231-786-5
MDL number:
MFCD00003390
Assay:
≥98%
Form:
powder
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grade

Molecular Biology

Quality Segment

200

vapor density

7.9 (vs air)

assay

≥98%

form

powder

reaction suitability

reagent type: oxidant

technique(s)

electrophoresis: suitable

anion traces

chloride (Cl-): <10 ppm

cation traces

Fe: <10 ppm, heavy metals (as Pb): <50 ppm

foreign activity

DNAse, none detected, Protease, none detected, RNAse, none detected

SMILES string

N.N.OS(=O)(=O)OOS(O)(=O)=O

InChI

1S/2H3N.H2O8S2/c;;1-9(2,3)7-8-10(4,5)6/h2*1H3;(H,1,2,3)(H,4,5,6)

InChI key

ROOXNKNUYICQNP-UHFFFAOYSA-N

General description

Ammonium persulfate (APS) is a widely used reagent in biochemistry and molecular biology for the preparation of polyacrylamide gels. APS forms oxygen free radicals in aqueous solution by a base-catalyzed mechanism. The bases, most commonly used as catalysts, are tertiary amines such as TEMED (N,N,N′,N′-tetramethylethylenediamine) or DMAPN (3-dimethylaminopropionitrile). The free radicals will cause the polymerization of acrylamide and bis-acrylamide to form a gel matrix, which can be used for separating macromolecules by size. APS has also been utilized to study protein-protein interactions via photoinitiated crosslinking chemistry.

Application

Catalyst for acrylamide gel polymerization.
Ammonium persulfate has been used for the preparation of polyacrylamide gels and acrylamide hydrogels.

Analysis Note

Tested for use in acrylamide polymerization.

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Ox. Sol. 3 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

5.1B - Oxidizing hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

Class I Designated Chemical Substances

prtr

Substances Subject to be Indicated Names

ishl_indicated

Substances Subject to be Notified Names

ishl_notified

A3678-25G:4.548173946955E12 + A3678-100G:4.548173946948E12

jan

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