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SMC® High Sensitivity Immunoassay Kits

SMC® technology enables high sensitivity measurement of biomarkers at femtogram levels, providing insight for early biology to clinical progression through the differentiation of healthy and disease states. This technology, combined with the FemtoQuest™ system or SMCxPRO® instrument and suite of immunoassay kits, provides researchers with the critical tools needed to accelerate their research.

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Digital Counting Improves Sensitivity and Dynamic Range

Providing maximum immunoassay performance while following a workflow similar to traditional ELISA technology, SMC® technology combines a unique assay elution step and robust digital counting to achieve improved signal-to-noise ratios over traditional immunoassay technologies. The SMC® technology thus provides enhanced quantification at both low and high levels of expression on one complete system. The FemtoQuest™ system and SMCxPRO® instrument capture the sum of all digital events counted above the background threshold. The digital event algorithm of the FemtoQuest™ system and SMCxPRO® instrument computes digital events across a spectrum of time series for a single standard. The digital counting of molecules thus improves the assay sensitivity and extends the assay dynamic range beyond what can be achieved with traditional methods.

Verification Criteria for SMC® Immunoassays

A comprehensive set of criteria evaluating ultimate performance are used to qualify verified SMC® immunoassays, including:

  • Lower limit of quantification
  • Inter- and intra-assay precision
  • Spike recovery
  • Dilutional linearity and parallelism

Related Resources

Learn more about how SMC® technology can take your research to the next level by exploring the topics below.

SMC® Research Areas

Browse all the ways researchers are using SMC® technology including drug development, neuroscience, cardiotoxicity, immunology research, and more.

Browse Research Areas >

Why Choose SMC® Technology?

See why choosing ultrasensitive SMC® technology can help you take a deeper look at your research to discover even more.

See Why >

SMC® Instrument Platforms

The FemtoQuest™ system, with SMC® assays, enables the measurement of previously undetectable biomarkers. Learn more about our ultrasensitive platforms.

Discover More >

Other SMC® Instrumentation

View info on ancillary kits and equipment for SMC® assays that help optimize your workflows.

View Equipment >

Custom Assay Services

Explore more about our Custom Assay Development & Innovation (CADI) services and request more info.

Explore Services >

Tips and Support for SMC® Products

Get tips and tricks for running SMC® assays straight from the experts.

Get Tips & Tricks >

Subscribe to Our Newsletter

Stay up to date on the latest immunoassay research trends and our newest product offerings by subscribing to our Analyte Update newsletter or explore our current immunoassay offerings in the Analyte Quarterly.

Related Videos

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How to Seal Read Plates for SMC<sup>®</sup> Assays
How to Seal Read Plates for SMC® Assays
Learn how to properly seal the 384-well read plates for the FemtoQuest™ or SMCxPRO® platform in four easy steps.
How to Pipette for Successful Assays
How to Pipette for Successful Assays
Get expert tips for forward and reverse pipetting for the most successful SMC® ultrasensitive assays.
SMCxPRO<sup>®</sup> Instrument Set-up and Calibration
SMCxPRO® Instrument Set-up and Calibration
Learn how to initialize and calibrate your SMCxPRO® instrument.
How to Reconstitute the IL-6 Standard
How to Reconstitute the IL-6 Standard
Learn how to reconstitute the IL-6 standard for the SMC®platform.
How to Prepare the IL-6 Standard Curve
How to Prepare the IL-6 Standard Curve
Discover how to prepare the IL-6 standard curve using SMC® technology.
How to Prepare and Dispense the IL-6 Beads
How to Prepare and Dispense the IL-6 Beads
Watch how to prepare and dispense the IL-6 beads for SMC® assays.
How to Prepare and Dispense the Detection Antibody
How to Prepare and Dispense the Detection Antibody
Watch how to prepare and dispense the detection antibody for SMC® assays.
Elution and Neutralization Steps
Elution and Neutralization Steps
View key elution and neutralization steps in the SMC® protocol.
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